Inhibition of 4NQO-Induced Oral Carcinogenesis by Dietary Oyster Shell Calcium.

Abstract

Oyster has gained much attention recently for its anticancer activity but it is unclear whether calcium, the major antitumor ingredient in oyster shell, is responsible for the anticarcinogenic role of the oyster. To address this issue, C57BL/6 mice were fed with the carcinogen 4-nitroquinoline-1-oxide (4NQO, 50 µg/mL) and normal diet or a diet containing oyster powder, oyster calcium, or calcium depleted oyster powder. The tongue tissue specimens isolated from these mice were histologically evaluated for hyperplasia, dysplasia, and papillary lesions, and then analyzed for proliferation and differentiation markers by immunohistochemistry. The results showed that mice on the diet containing oyster calcium significantly reduced rates of tumors in the tongue and proliferation and enhanced differentiation in the oral epithelium compared with the diet containing calcium depleted oyster powder. These results suggest that calcium in oyster plays a critical role in suppressing formation of oral squamous cell carcinoma and proliferation and promoting differentiation of the oral epithelium.

• Authors: Ying Chen, Yi Jiang, Liyan Liao, Xiaoxin Zhu, Shengan Tang, Qing Yang, Lihua Sun, Yujie Li, Shuangrong Gao, Zhongjian Xie
• Journal: Integrative cancer therapies (Integr Cancer Ther) Vol. 15 Issue 1 Pg. 96-101 (Mar 2016) ISSN: 1552-695X [Electronic] United States
• PMID: 26293805 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2015.

Chemical References

• Antineoplastic Agents
• Carcinogens
• 4-Nitroquinoline-1-oxide
• Calcium

Topics

• 4-Nitroquinoline-1-oxide (adverse effects)
• Animal Shells (chemistry)
• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Calcium (pharmacology)
• Carcinogenesis (drug effects)
• Carcinogens (toxicity)
• Carcinoma, Squamous Cell (chemistry, drug therapy)
• Cell Differentiation (drug effects)
• Cell Proliferation (drug effects)
• Epithelium (drug effects)
• Female
• Male
• Mice
• Mice, Inbred C57BL
• Mouth Neoplasms (chemically induced, drug therapy)
• Ostreidae (chemistry)
• Tongue (drug effects)