Abstract | BACKGROUND: Metabolic derangement is a key hallmark of major traumatic injury. The recent introduction of mass spectrometry-based metabolomics technologies in the field of trauma shed new light on metabolic aberrations in plasma that are triggered by trauma and hemorrhagic shock. Alteration in metabolites associated with catabolism, acidosis and hyperglycemia have been identified. However, the mechanisms underlying fluxes driving such metabolic adaptations remain elusive. METHODS: A bolus of U-(13)C-glucose was injected in Sprague-Dawley rats at different time points. Plasma extracts were analyzed via ultra-high performance liquid chromatography-mass spectrometry to detect quantitative fluctuations in metabolite levels as well as to trace the distribution of heavy labeled carbon isotopologues. RESULTS: CONCLUSIONS: These preliminary results are consistent with observations we have recently obtained in humans, and expand upon our early results on rodent models of trauma and hemorrhagic shock by providing the kinetics of glucose fluxes after trauma and hemorrhage. Despite the preliminary nature of this study, owing to the limited number of biological replicates, results highlight a role for shock, rather than trauma alone, in eliciting systemic metabolic aberrations. This study provides the foundation for tracing experiments in rat models of trauma. The goal is to improve our understanding of substrate specific metabolic derangements in trauma/ hemorrhagic shock, so as to design resuscitative strategies tailored toward metabolic alterations and the severity of trauma.
|
Authors | Angelo D'Alessandro, Annie L Slaughter, Erik D Peltz, Ernest E Moore, Christopher C Silliman, Matthew Wither, Travis Nemkov, Anthony W Bacon, Miguel Fragoso, Anirban Banerjee, Kirk C Hansen |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 13
Pg. 253
(Aug 05 2015)
ISSN: 1479-5876 [Electronic] England |
PMID | 26242576
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Animals
- Carbon
(metabolism)
- Glycolysis
- Isotope Labeling
(methods)
- Lactic Acid
(blood)
- Metabolic Flux Analysis
- Metabolomics
(methods)
- Rats, Sprague-Dawley
- Shock, Hemorrhagic
(blood, metabolism)
- Wounds and Injuries
(blood, metabolism)
|