Expression of hypoxia-inducible factor-1α during ovarian follicular growth and development in Sprague-Dawley rats.
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| Abstract |
Hypoxia-inducible factor-1α (HIF-1α) has been identified as a transcription factor that is involved in diverse physiological and pathological processes in the ovary. In this study, we examined whether HIF-1α is expressed in a cell- and stage-specific manner during follicular growth and development in the mammalian ovaries. Using immunohistochemistry and Western blot analysis, HIF-1α expression was observed in granulosa cells specifically and was significantly increased during the follicular growth and development of postnatal rats. Furthermore, pregnant mare serum gonadotropin also induced HIF-1α expression in granulosa cells and ovaries during the follicular development of immature rats primed with gonadotropin. Moreover, we also examined proliferation cell nuclear antigen, a cell proliferation marker, during follicular growth and development and found that its expression pattern was similar to that of HIF-1α protein. Granulosa cell culture experiments revealed that proliferation cell nuclear antigen expression may be regulated by HIF-1α. These results indicated that HIF-1α plays an important role in the follicular growth and development of these 2 rat models. The HIF-1α-mediated signaling pathway may be an important mechanism regulating follicular growth and development in mammalian ovaries in vivo.
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| Authors | Z H Zhang, L Y Chen, F Wang, Y Q Wu, J Q Su, X H Huang, Z C Wang, Y Cheng |
| Journal | Genetics and molecular research : GMR (Genet Mol Res) Vol. 14 Issue 2 Pg. 5896-909 (Jun 01 2015) ISSN: 1676-5680 [Electronic] Brazil |
| PMID | 26125789 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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