The role of Cyclin G1 in cellular proliferation and apoptosis of human epithelial ovarian cancer.

Abstract

Cyclin G1 plays an essential role in the development of human carcinoma. Here, we characterized the clinical significance of Cyclin G1 and investigated its role in cellular proliferation and apoptosis of epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of Cyclin G1 in nine fresh EOC tissues and three fresh normal ovarian tissues. Immunohistochemistry analysis was performed on formalin-fixed paraffin-embedded section of 119 cases of EOCs. Using cell counting kit (CCK)-8 and colony formation assays, we analyzed the effect of Cyclin G1 in cellular proliferation of EOC. Besides, the immunofluorescence and flow cytometry analysis was performed to study the role of Cyclin G1 in cellular apoptosis of EOC. We found Cyclin G1 was up-regulated in EOC tissues compared with the normal ovary tissues. Cyclin G1 expression in EOC was closely correlated with differentiation grade (P = 0.009) and malignant tumor cells in ascites (P = 0.009). The Kaplan-Meier curve showed that higher expression of Cyclin G1 was associated with significantly shorter survival in EOC patients. Multivariate analysis suggested Cyclin G1 expression was an independent prognostic factor for overall survival. CCK-8 and colony formation assays revealed that depletion of Cyclin G1 inhibited the proliferation and clone formation. Combined immunofluorescence and flow cytometry analysis showed that silencing of Cyclin G1 with shRNA could promote apoptosis of ovarian cancer cells. Additionally, the result of immunoprecipitation test showed Cyclin G1 interacted with CDK2 in EOC cells. In summary, our findings suggest that Cyclin G1 may be involved in the prognosis of EOC patients and be a useful therapeutic target for EOC.

• Authors: Lifei Jiang, Rong Liu, Yingying Wang, Chunmiao Li, Qinghua Xi, Jianxin Zhong, Jian Liu, Shuyun Yang, Juan Wang, Menghui Huang, Chunhui Tang, Zheng Fang
• Journal: Journal of molecular histology (J Mol Histol) Vol. 46 Issue 3 Pg. 291-302 (Jun 2015) ISSN: 1567-2387 [Electronic] Netherlands
• PMID: 25981880 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCNG1 protein, human
• Cyclin G1
• TP53 protein, human
• Tumor Suppressor Protein p53
• CDK2 protein, human
• Cyclin-Dependent Kinase 2

Topics

• Adult
• Aged
• Aged, 80 and over
• Apoptosis
• Carcinoma, Ovarian Epithelial
• Cell Line, Tumor
• Cell Proliferation
• Cyclin G1 (physiology)
• Cyclin-Dependent Kinase 2 (metabolism)
• Female
• Gene Expression
• Humans
• Kaplan-Meier Estimate
• Middle Aged
• Neoplasms, Glandular and Epithelial (metabolism, mortality, pathology)
• Ovarian Neoplasms (metabolism, mortality, pathology)
• Proportional Hazards Models
• Tumor Suppressor Protein p53 (metabolism)
• Young Adult