1H-NMR-based metabolomic analysis of cerebrospinal fluid from adult bilateral moyamoya disease: comparison with unilateral moyamoya disease and atherosclerotic stenosis.

Abstract

Although metabolomics has been increasingly used to observe metabolic pattern and disease-specific metabolic markers, metabolite profiling for moyamoya disease (MMD) has not yet been done in adults. This study investigated cerebrospinal fluid (CSF) metabolites specific to bilateral MMD (B-MMD) and compared them to those of unilateral MMD (U-MMD) or atherosclerotic stenosis with hydrogen-1 nuclear magnetic resonance spectroscopy to identify metabolic biomarkers associated with MMD in adults.CSF samples of B-MMD (n = 29), U-MMD (n = 11), and atherosclerotic cerebrovascular disease (ACVD) (n = 8) were recruited. Principal component analysis, partial least square discriminant analysis, and orthogonal projections to latent structure discriminant analysis (OPLS-DA) were done for the comparisons. Diagnostic performance was acquired by prediction of 1 left-out sample from the distinction model constructed with the rest of the samples.B-MMD showed an increase in glutamine (P < 0.001) and taurine (P = 0.004), and a decrease in glucose (P < 0.001), citrate (P = 0.002), and myo-inositol (P = 0.006) than those in ACVD. U-MMD showed a higher level of glutamine (P = 0.005) and taurine (P = 0.034), and a lower level of glutamate (P < 0.004) than those in ACVD. No difference at the metabolite level was observed between B-MMD and U-MMD. Cross-validation with the OPLS-DA model showed a high accuracy for the prediction of MMD.The results of the study suggest that a metabolomics approach may be helpful in confirming MMD and providing a better understanding of MMD pathogenesis. Elevated glutamine in the CSF may be associated with MMD pathogenesis, which was different from ACVD.

• Authors: Jin Pyeong Jeon, Taeho Yun, Xing Jin, Won-Sang Cho, Young-Je Son, Jae Seung Bang, Hyun-Seung Kang, Chang Wan Oh, Jeong Eun Kim, Sunghyouk Park
• Journal: Medicine (Medicine (Baltimore)) Vol. 94 Issue 17 Pg. e629 (May 2015) ISSN: 1536-5964 [Electronic] United States
• PMID: 25929894 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Glutamine
• Taurine
• Citric Acid
• Glutamic Acid
• Inositol
• Glucose

Topics

• Adult
• Biomarkers (cerebrospinal fluid)
• Citric Acid (cerebrospinal fluid)
• Female
• Glucose (cerebrospinal fluid)
• Glutamic Acid (cerebrospinal fluid)
• Glutamine (cerebrospinal fluid)
• Humans
• Inositol (cerebrospinal fluid)
• Intracranial Arteriosclerosis (cerebrospinal fluid)
• Magnetic Resonance Spectroscopy (methods)
• Male
• Metabolomics (methods)
• Middle Aged
• Moyamoya Disease (cerebrospinal fluid)
• Prospective Studies
• Taurine (cerebrospinal fluid)