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[Cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy].

Abstract
To develop a cell-penetrating chimeric apoptotic peptide AVPI-LMWP/DNA co-delivery system for cancer therapy, we prepared the AVPI-LMWP/pTRAIL self-assembled complexes containing a therapeutic combination of peptide drug AVPI and DNA drug TRAIL. The chimeric apoptotic peptide AVPI-LMWP was synthesized using the standard solid-phase synthesis. The cationic AVPI-LMWP could condense pTRAIL by electrostatic interaction. The physical-chemical properties of the AVPI-LMWP/pTRAIL complexes were characterized. The cellular uptake efficiency and the inhibitory activity of the AVPI-LMWP/pTRAIL complexes on tumor cell were also performed. The results showed that the AVPI-LMWP/pTRAIL complexes were successfully prepared by co-incubation. With the increase of mass ratio (AVPI-LMWP/DNA), the particle size was decreased and the zeta potential had few change. Agarose gel electrophoresis showed that AVPI-LMWP could fully bind and condense pTRAIL at a mass ratio above 15:1. Cellular uptake efficiency was improved along with the increased ratio of W(AVPI-LMWP)/WpTRAIL. The in vitro cytotoxicity experiments demonstrated that the AVPI-LMWP/pTRAIL (W:W = 20:1) complexes was significantly more effective than the pTRAIL, AVPI-LMWP alone or LMWP/pTRAIL complexes on inhibition of HeLa cell growth. Our studies indicated that the AVPI-LMWP/pTRAIL co-delivery system could deliver plasmid into HeLa cell and induce tumor cell apoptosis efficiently, which showed its potential in cancer therapy using combination of apoptoic peptide and gene drugs.
AuthorsJiao Tan, Ya-Ping Wang, Hui-Xin Wang, Jian-Ming Liang, Meng Zhang, Xun Sun, Yong-Zhuo Huang
JournalYao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 49 Issue 12 Pg. 1718-23 (Dec 2014) ISSN: 0513-4870 [Print] China
PMID25920203 (Publication Type: Journal Article)
Chemical References
  • Antineoplastic Agents
  • Cell-Penetrating Peptides
  • DNA
Topics
  • Antineoplastic Agents (chemistry)
  • Cell-Penetrating Peptides (chemistry)
  • DNA (chemistry)
  • Drug Delivery Systems
  • HeLa Cells
  • Humans
  • Neoplasms (drug therapy)
  • Particle Size
  • Plasmids

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