Abstract |
To develop a cell-penetrating chimeric apoptotic peptide AVPI-LMWP/ DNA co-delivery system for cancer therapy, we prepared the AVPI-LMWP/pTRAIL self-assembled complexes containing a therapeutic combination of peptide drug AVPI and DNA drug TRAIL. The chimeric apoptotic peptide AVPI-LMWP was synthesized using the standard solid-phase synthesis. The cationic AVPI-LMWP could condense pTRAIL by electrostatic interaction. The physical-chemical properties of the AVPI-LMWP/pTRAIL complexes were characterized. The cellular uptake efficiency and the inhibitory activity of the AVPI-LMWP/pTRAIL complexes on tumor cell were also performed. The results showed that the AVPI-LMWP/pTRAIL complexes were successfully prepared by co-incubation. With the increase of mass ratio (AVPI-LMWP/ DNA), the particle size was decreased and the zeta potential had few change. Agarose gel electrophoresis showed that AVPI-LMWP could fully bind and condense pTRAIL at a mass ratio above 15:1. Cellular uptake efficiency was improved along with the increased ratio of W(AVPI-LMWP)/WpTRAIL. The in vitro cytotoxicity experiments demonstrated that the AVPI-LMWP/pTRAIL (W:W = 20:1) complexes was significantly more effective than the pTRAIL, AVPI-LMWP alone or LMWP/pTRAIL complexes on inhibition of HeLa cell growth. Our studies indicated that the AVPI-LMWP/pTRAIL co-delivery system could deliver plasmid into HeLa cell and induce tumor cell apoptosis efficiently, which showed its potential in cancer therapy using combination of apoptoic peptide and gene drugs.
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Authors | Jiao Tan, Ya-Ping Wang, Hui-Xin Wang, Jian-Ming Liang, Meng Zhang, Xun Sun, Yong-Zhuo Huang |
Journal | Yao xue xue bao = Acta pharmaceutica Sinica
(Yao Xue Xue Bao)
Vol. 49
Issue 12
Pg. 1718-23
(Dec 2014)
ISSN: 0513-4870 [Print] China |
PMID | 25920203
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Cell-Penetrating Peptides
- DNA
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Topics |
- Antineoplastic Agents
(chemistry)
- Cell-Penetrating Peptides
(chemistry)
- DNA
(chemistry)
- Drug Delivery Systems
- HeLa Cells
- Humans
- Neoplasms
(drug therapy)
- Particle Size
- Plasmids
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