Abstract | OBJECTIVE: METHODS: The mRNA levels of LC3 in PBMCs from 56 SLE patients and 45 healthy individuals were detected by real-time quantitative polymerase chain reaction (qPCR) technique. Autophagy in PBMCs was also determined by flow cytometry (FACs) in 20 SLE patients and 15 healthy controls. The correlation between LC3 mRNA expression and disease activity of SLE (SLEDAI) was then analyzed. RESULTS: The mRNA level of LC3 (RQ) in SLE patients was obviously downregulated compared with that in healthy population (1.30 ± 0.10 vs 1.35 ± 0.09; P = 0.029), paralleled with the decreased autophagy rate detected by flow cytometry in PBMCs of SLE patients [(2.21 ± 1.07) % vs (9.91 ± 4.01) %;P = 0.047]. Moreover, LC3 mRNA expression level was negatively correlated with SLEDAI (r = -0.337, P = 0.023). However, when the clinical features of 27 SLE patients with decreased LC3 mRNA expression (RQ<1.351) were compared with those of other 29 SLE patients with normal or high LC3 mRNA expression (RQ>1.351), increasing rates of arthritis, serositis, hematological abnormalities were noted in patients with decreased LC3 mRNA expression yet without statistically significance. However, there was a significant difference between two groups in the incidence of renal involvement (P = 0.028). CONCLUSION: The impaired autophagy due to downregulated LC3 mRNA level in SLE patients indicates that autophagy plays a role in mediating the occurrence and development of SLE.
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Authors | Xiongyan Luo, Minghui Yang, Yanhui Xia, Yang Xiang, Yi Liu, Guohua Yuan |
Journal | Zhonghua nei ke za zhi
(Zhonghua Nei Ke Za Zhi)
Vol. 54
Issue 2
Pg. 134-8
(Feb 2015)
ISSN: 0578-1426 [Print] China |
PMID | 25907845
(Publication Type: Journal Article)
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Chemical References |
- Microtubule-Associated Proteins
- RNA, Messenger
|
Topics |
- Down-Regulation
- Flow Cytometry
- Humans
- Leukocytes, Mononuclear
(cytology, metabolism)
- Lupus Erythematosus, Systemic
(blood)
- Microtubule-Associated Proteins
(blood, metabolism)
- RNA, Messenger
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