[Expression of prostaglandin transporter in colorectal cancer tissues and its relationship with clinicopathological features].

Abstract

OBJECTIVE:

To investigate the expression of prostaglandin transporter (PGT) in colorectal cancer (CRC) tissues and its relationship with clinicopathological features.

METHODS:

The mRNA and protein levels of PGT were determined by real-time PCR, Western blot and immunohistochemical methods in cancer tissues and adjacent normal tissue from 80 patients with colorectal cancer and their relationship with clinicopathological features was analyzed.

RESULTS:

Compared with the adjacent normal tissue of colorectal cancer, the PGT mRNA relative expression (0.57 ± 0.33 vs. 2.33 ± 1.20) and the PGT protein expression in cancer tissues decreased significantly [PGT/GAPDH 0.45 ± 0.16 vs. 0.78 ± 0.23, integral A 718.7 ± 359.4 vs. 10412.0 ± 6423.3, average A 0.03 ± 0.01 vs. 0.12 ± 0.09, all P<0.01]. Lower mRNA and protein expressions of PGT in colorectal cancer were associated with depth of invasion T3 to T4 and TNM stage III( to IIII( (P<0.01), while not associated with gender, age, tumor location and differentiation degree (all P>0.05).

CONCLUSION:

Expression levels of PGT mRNA and protein in colorectal cancer tissue are significantly down-regulation. PGT expression is associated with invasion depth and late stages.

• Authors: Shanliang Shang, Xiujun Liao, Zhong Shen, Jianming Qiu, Shuxian Shao, Lie Ding, Dong Wang, Guangen Yang, Yanxiang Zhang
• Journal: Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery (Zhonghua Wei Chang Wai Ke Za Zhi) Vol. 18 Issue 3 Pg. 277-81 (Mar 2015) ISSN: 1671-0274 [Print] China
• PMID: 25809334 (Publication Type: Journal Article)

Chemical References

• Organic Anion Transporters
• RNA, Messenger
• SLCO2A1 protein, human

Topics

• Colorectal Neoplasms
• Down-Regulation
• Humans
• Neoplasm Invasiveness
• Neoplasm Staging
• Organic Anion Transporters
• RNA, Messenger