Evaluation of a Streptococcus thermophilus strain with innate anti-inflammatory properties as a vehicle for IL-10 cDNA delivery in an acute colitis model.

Abstract

The aim of this work was to develop a Streptococcus (S.) thermophilus strain with improved anti-inflammatory properties due to the incorporation of the therapeutic cDNA delivery plasmid pValac::il-10. To achieve this purpose, cells of S. thermophilus CRL807, previously selected as being an important anti-inflammatory strain, were electroporated with pValac::il-10 plasmid. In order to confirm the functionality of the developed strain, it was co-cultured with human epithelial cells Caco-2 and the production of IL-10 was evaluated by ELISA. Bacterial suspensions of S. thermophilus CRL807 containing pValac::il-10 plasmid or of the wild-type (WT) strain were administered in vivo using a murine model of intestinal inflammation. The animals treated with S. thermophilus CRL807 pValac::il-10 showed a lower body weight loss, microbial translocation to liver and damage scores in their intestines at macroscopical and microscopic levels. Furthermore, a significant increase was observed in the concentration of IL-10 in the intestinal contents of these mice compared to the rest of the experimental groups, accompanied by decreased levels of pro-inflammatory cytokines. The insertion of the therapeutic pValac::il-10 plasmid increased the intrinsic anti-inflammatory activity (synergetic effect) of S. thermophilus CRL807 which could be included in novel treatment protocols for inflammatory bowel diseases.

• Authors: Silvina del Carmen, Anderson Miyoshi, Vasco Azevedo, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc
• Journal: Cytokine (Cytokine) Vol. 73 Issue 2 Pg. 177-83 (Jun 2015) ISSN: 1096-0023 [Electronic] England
• PMID: 25777482 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• DNA, Complementary
• Interleukin-10

Topics

• Animals
• Anti-Inflammatory Agents (metabolism)
• Body Fluids (metabolism)
• Body Weight (drug effects)
• Caco-2 Cells
• Colitis (drug therapy, microbiology, pathology)
• DNA, Complementary (metabolism)
• Disease Models, Animal
• Drug Delivery Systems
• Humans
• Interleukin-10 (pharmacology, therapeutic use)
• Intestines (drug effects, pathology)
• Liver (drug effects, microbiology, pathology)
• Mice
• Streptococcus thermophilus (drug effects, metabolism)