18F-FPRGD2 PET/CT imaging of integrin αvβ3 in renal carcinomas: correlation with histopathology.

Abstract

UNLABELLED:

This study aimed to correlate (18)F-FB-mini-PEG-E[c(RGDyK)](2) ((18)F-FPRGD2) uptake to integrin αvβ3 expression and angiogenesis in renal tumors.

METHODS:

(18)F-FPRGD2 PET/CT was performed on 27 patients before surgical resection (median 4 d) of a renal mass. The (18)F-FPRGD2 uptake was compared with integrin αvβ3, CD31, CD105, and Ki-67 using immunohistochemistry; with placental growth factor and vascular endothelial growth factor receptors 1 and 2 using reverse transcription polymerase chain reaction; and with vascular endothelial growth factor A isoforms using enzyme-linked immunosorbent assay.

RESULTS:

Overall, (18)F-FPRGD2 uptake significantly correlated (P < 0.0001) with integrin αvβ3 expression in renal masses. However, it correlated only with integrin αvβ3-positive vessels in the group of papillary carcinomas whereas it correlated with integrin αvβ3 expression by tumor cells in the clear cell carcinoma group.

CONCLUSION:

(18)F-FPRGD2 uptake reflects the expression of integrin αvβ3 in renal tumors but represents angiogenesis only when tumor cells do not express the integrin.

• Authors: Nadia Withofs, Nicolas Signolle, Joan Somja, Pierre Lovinfosse, Eugène Mutijima Nzaramba, Frédéric Mievis, Fabrice Giacomelli, David Waltregny, Didier Cataldo, Sanjiv S Gambhir, Roland Hustinx
• Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (J Nucl Med) Vol. 56 Issue 3 Pg. 361-4 (Mar 2015) ISSN: 1535-5667 [Electronic] United States
• PMID: 25655629 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 by the Society of Nuclear Medicine and Molecular Imaging, Inc.

Chemical References

• 4-fluorobenozyl-glutamyl-cyclo(arginyl-glycyl-aspartyl-tyrosyl-lysyl)2
• Integrin alphaVbeta3
• Peptides, Cyclic
• Platelet Endothelial Cell Adhesion Molecule-1
• Radiopharmaceuticals
• Polyethylene Glycols

Topics

• Aged
• Carcinoma, Renal Cell (diagnostic imaging)
• Cell Membrane (metabolism)
• Female
• Humans
• Integrin alphaVbeta3 (metabolism)
• Kidney Neoplasms (diagnostic imaging)
• Male
• Microcirculation
• Middle Aged
• Neovascularization, Pathologic
• Nuclear Medicine
• Peptides, Cyclic
• Platelet Endothelial Cell Adhesion Molecule-1 (metabolism)
• Polyethylene Glycols (chemistry)
• Prospective Studies
• Radionuclide Imaging
• Radiopharmaceuticals
• Tomography, X-Ray Computed
• Treatment Outcome