Myricitrin alleviates MPP⁺-induced mitochondrial dysfunction in a DJ-1-dependent manner in SN4741 cells.

Abstract

Oxidative stress and mitochondrial dysfunction have been linked to Parkinson's disease. DJ-1 is a recessive familial PD gene involved in antioxidative function and mitochondrial maintenance. Myricitrin, a flavanoid isolated from the root bark of Myrica cerifera, has potent antioxidative properties. In the present study, we investigated the protective effects of myricitrin against MPP(+)-induced mitochondrial dysfunction in SN4741 cells and attempted to elucidate the mechanisms underlying this protection. The results showed that incubating SN4741 cells with myricitrin significantly reduced cell death induced by the neurotoxin MPP(+). Furthermore, myricitrin protected cells from MPP(+)-induced effects on mitochondrial morphology and function. However, these protective effects were lost under DJ-1-deficient conditions. Thus, our results suggest that myricitrin alleviates MPP(+)-induced mitochondrial dysfunction and increases cell viability via DJ-1, indicating that myricitrin is a potential beneficial agent for age-related neurodegenerative diseases, particularly Parkinson's disease.

• Authors: Zhibiao Cai, Weijun Zeng, Kai Tao, Fangfang Lu, Guodong Gao, Qian Yang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 458 Issue 2 Pg. 227-33 (Mar 06 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 25623535 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Flavonoids
• Neuroprotective Agents
• Neurotoxins
• Oncogene Proteins
• myricitrin
• Peroxiredoxins
• PARK7 protein, mouse
• Protein Deglycase DJ-1
• 1-Methyl-4-phenylpyridinium

Topics

• 1-Methyl-4-phenylpyridinium (administration & dosage)
• Animals
• Antioxidants (administration & dosage)
• Apoptosis (drug effects, physiology)
• Cell Line
• Cell Survival (drug effects, physiology)
• Dose-Response Relationship, Drug
• Drug Interactions
• Flavonoids (administration & dosage)
• Mice
• Mitochondria (drug effects, physiology)
• Neurons (drug effects, physiology)
• Neuroprotective Agents (administration & dosage)
• Neurotoxins (administration & dosage)
• Oncogene Proteins (metabolism)
• Peroxiredoxins (metabolism)
• Protein Deglycase DJ-1