Anti-metastatic effect and mechanisms of Wenshen Zhuanggu Formula in human breast cancer cells.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Wenshen Zhuanggu Formula (WSZG), a traditional Chinese medicine (TCM) empirical prescription, has been used to treat the patients with breast cancer bone metastasis as an adjuvant in clinical practice. To explore the anti-metastatic activity and potential mechanisms of WSZG-containing serum (WSZG-CS) on highly bone-metastatic human breast cancer MDA-MB-231BO cells.

MATERIALS AND METHODS:

MDA-MB-231BO cells were cultured alone or co-cultured with bone marrow-derived mesenchymal stem cells (BMSCs). Invasion assays were carried out in Matrigel-coated Transwell chambers. CC chemokine 5 (CCL5) and interleukin (IL)-17B secretion levels were detected by ELISA. CCR5 and IL-17BR protein expression levels were determined by immunocytochemistry and Western blot analysis.

RESULTS:

Compared with control serum, WSZG-CS significantly inhibited BMSC induced MDA-MB-231BO breast cancer cell invasion, reduced CCL5 and IL-17B levels in co-culture supernatants, and downregulated CCR5 and IL-17BR protein expression in breast cancer cells co-cultured with BMSCs.

CONCLUSIONS:

WSZG-CS exerts an anti-metastatic activity against MDA-MB-231BO breast cancer cells, due to its ability to mitigate the interaction between BMSCs and breast cancer cells mediated via the CCL5/CCR5 and IL-17B/IL-17BR signaling pathways.

• Authors: Xiang-hui Han, Chun-li Wang, Yan Xie, Jiang Ma, Xiao-hui Zhang, Qian-wen Hu, Ge Lin, Sheng Liu, Jin-gen Lu
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 162 Pg. 39-46 (Mar 13 2015) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 25554638 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• CCL5 protein, human
• Chemokine CCL5
• Coumarins
• Furocoumarins
• Interleukin-17
• Plant Extracts
• Wenshen Zhuanggu
• angelicin
• Ficusin
• osthol

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Breast Neoplasms (metabolism)
• Cell Line
• Cell Line, Tumor
• Chemokine CCL5 (metabolism)
• Coculture Techniques
• Coumarins (analysis)
• Female
• Ficusin (analysis)
• Furocoumarins (analysis)
• Humans
• Interleukin-17 (metabolism)
• Male
• Medicine, Chinese Traditional
• Mesenchymal Stem Cells (metabolism)
• Plant Extracts (chemistry, pharmacology)
• Rats, Sprague-Dawley
• Serum