Angiomotin is a novel component of cadherin-11/β-catenin/p120 complex and is critical for cadherin-11-mediated cell migration.

Abstract

Loss of E-cadherin and up-regulation of mesenchymal cadherins, a hallmark of the epithelial-mesenchymal transition, contributes to migration and dissemination of cancer cells. Expression of human cadherin-11 (Cad11), also known as osteoblast cadherin, in prostate cancer increases the migration of prostate cancer cells. How Cad11 mediates cell migration is unknown. Using the human Cad11 cytoplasmic domain in pulldown assays, we identified human angiomotin (Amot), known to be involved in cell polarity, migration, and Hippo pathway, as a component of the Cad11 protein complex. Deletion analysis showed that the last C-terminal 10 amino acids in Cad11 cytoplasmic domain are required for Amot binding. Further, Cad11 preferentially interacts with Amot-p80 than Amot-p130 isoform and binds directly to the middle domain of Amot-p80. Cad11-Amot interaction affects Cad11-mediated cell migration, but not homophilic adhesion, as deletion of Amot binding motif of Cad11 (Cad11-ΔAmot) did not abolish Cad11-mediated cell-cell adhesion of mouse L cells, but significantly reduced Cad11-mediated cell migration of human C4-2B4 and PC3-mm2 prostate cancer cells and human HEK293T cells. Together, our studies identified Amot-p80 as a novel component of the Cad11 complex and demonstrated that Amot-p80 is critical for Cad11-mediated cell migration.

• Authors: Angelica Ortiz, Yu-Chen Lee, Guoyu Yu, Hsuan-Chen Liu, Song-Chang Lin, Melmet Asim Bilen, Hyojin Cho, Li-Yuan Yu-Lee, Sue-Hwa Lin
• Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (FASEB J) Vol. 29 Issue 3 Pg. 1080-91 (Mar 2015) ISSN: 1530-6860 [Electronic] United States
• PMID: 25466890 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: © FASEB.

Chemical References

• AMOT protein, human
• Angiomotins
• CTNNB1 protein, human
• Cadherins
• Intercellular Signaling Peptides and Proteins
• Membrane Proteins
• Microfilament Proteins
• Protein Isoforms
• RNA, Messenger
• beta Catenin
• p120 GTPase Activating Protein
• osteoblast cadherin

Topics

• Amino Acid Sequence
• Angiomotins
• Animals
• Blotting, Western
• Cadherins (genetics, metabolism)
• Cell Adhesion
• Cell Movement
• Cell Proliferation
• Cells, Cultured
• Fluorescent Antibody Technique
• HEK293 Cells
• Humans
• Immunoenzyme Techniques
• Immunoprecipitation
• Intercellular Signaling Peptides and Proteins (genetics, metabolism)
• Male
• Membrane Proteins (genetics, metabolism)
• Mice
• Microfilament Proteins
• Molecular Sequence Data
• Prostatic Neoplasms (genetics, metabolism, pathology)
• Protein Isoforms
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Homology, Amino Acid
• beta Catenin (genetics, metabolism)
• p120 GTPase Activating Protein (genetics, metabolism)