Dental adhesives are necessary for the retention of specific dental restorations utilized to repair the anatomy of the tooth after
dental decay is removed. Adhesives come into contact with healthy and diseased periodontal tissues. Porphyromonas gingivalis is a gram negative bacterial pathogen, and
lipopolysaccharide (LPS-PG) is an
endotoxin found in gingival connective tissues of patients who suffer from
periodontal disease. The presence of the
endotoxin causes
inflammation. This study aims to evaluate the effectiveness of potent
dental adhesives when human gingival fibroblasts are challenged with LPS-PG. The fibroblasts were exposed to the
dental adhesives polymethly
methacrylate (
PMMA), OptiBond®, and Prime & Bond® which were purchased from Patterson Dental, a national
dental materials supplier. The human gingival fibroblasts (HGF-1, ATCC® CRL-2014) were purchased from American Type Culture Collection (ATCC). The porphyromonas gingival
lipopolysaccharide (LPS-PG) was purchased from Fisher Scientific (Pittsburg, PA). No significant differences in metabolic behavior was detected among the groups (p<0.132). While the
glutathione assay determined that there was not any significant increase in oxidative stress levels; the
lactate dehydrogenase assay identified significant cellular damage in the group exposed to combinations of the Prime & Bond® adhesives and LPS-PG at 48 hour intervals (p<0.003). No significant changes were noted in cellular morphology at any phases, and all cells demonstrated typical fibroblast spindle shape.