Abstract | AIM: METHODS:
Uterine fibroid tissues (UFC) and adjacent healthy uterine smooth muscle tissues (SMC) were collected from 30 cases of uterine fibroids. Real-time quantitative PCR and western blot were used to detect cell membrane calcium channel protein subtypes: TRPC1, TRPC3, TRPC4, TRPC6, TRPM6 and TRPM7. The effects of genes exhibiting most-notable differences on cell proliferation were examined using gene interference techniques. RESULTS: We found that calcium channel protein subtypes expressed differently in fibroids and the surrounding smooth muscles. The mRNA and protein expressions of TRPC1 and TRPM7 were higher in uterine fibroid tissues than in smooth muscle (P < 0.05), while no obvious difference was found in terms of other subtypes (TRPC3, TRPC4, TRPC6 and TRPM6). In cultured uterine leiomyoma cells, modifying the expressions of TRPC1 and TRPM7 significantly affected the proliferation rate of uterine fibroids. CONCLUSION:
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Authors | Xiaoping Ke, Zhongping Cheng, Xiaoyan Qu, Hong Dai, Wenchao Zhang, Zi-Jiang Chen |
Journal | International journal of clinical and experimental medicine
(Int J Clin Exp Med)
Vol. 7
Issue 5
Pg. 1324-30
( 2014)
ISSN: 1940-5901 [Print] United States |
PMID | 24995090
(Publication Type: Journal Article)
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