Abstract |
Thyroid cancer is the most common endocrine malignancy, and its incidence has increased rapidly worldwide. The molecular mechanisms underlying thyroid cancer tumorigenesis still need to be further investigated. MicroRNAs ( miRNAs), short RNA molecules of approximately 22 nucleotides in length, play crucial roles in tumorigenesis. In the present study, we found that the expression of miR-144 was significantly down-regulated in thyroid cancer as compared with that in normal thyroid tissues, suggesting that miR-144 may be involved in thyroid cancer tumorigenesis. Moreover, our results showed that restoration of miR-144 in K1 and WRO thyroid cancer cells could suppress the invasion and migration capability of these cells. We also demonstrated that miR-144 suppressed the expression of ZEB1 and ZEB2, two E-cadherin suppressors, by directly binding to their 3'-untranslated regions. Furthermore, restoration of ZEB1 or ZEB2 partially rescued the miR-144-induced inhibition of cell invasion. These data suggest miR-144 function as a tumor suppressor in thyroid cancer.
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Authors | Hongyu Guan, Weiwei Liang, Zhiwei Xie, Hai Li, Juan Liu, Liehua Liu, Lingling Xiu, Yanbing Li |
Journal | Endocrine
(Endocrine)
Vol. 48
Issue 2
Pg. 566-74
(Mar 2015)
ISSN: 1559-0100 [Electronic] United States |
PMID | 24968735
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Homeodomain Proteins
- MIRN144 microRNA, human
- MicroRNAs
- Repressor Proteins
- Transcription Factors
- ZEB1 protein, human
- ZEB2 protein, human
- Zinc Finger E-box Binding Homeobox 2
- Zinc Finger E-box-Binding Homeobox 1
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Topics |
- Cell Line, Tumor
- Down-Regulation
- Homeodomain Proteins
(metabolism)
- Humans
- MicroRNAs
(metabolism)
- Neoplasm Invasiveness
- Repressor Proteins
(metabolism)
- Thyroid Gland
(metabolism)
- Thyroid Neoplasms
(metabolism, pathology)
- Transcription Factors
(metabolism)
- Zinc Finger E-box Binding Homeobox 2
- Zinc Finger E-box-Binding Homeobox 1
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