Dynamics of cocaine- and amphetamine-regulated transcript containing cell changes in the adrenal glands of two kidney, one clip rats.

Abstract

Taking into consideration the homeostatic disorders resulting from renal hypertension and the essential role of cocaine- and amphetamine-regulated transcript (CART) in maintaining homeostasis by regulating many functions of the body, the question arises as to what extent the renovascular hypertension affects the morphology and dynamics of changes of CART-containing cells in the adrenal glands. The aim of the present study was to examine the distribution, morphology, and dynamics of changes of CART-containing cells in the adrenal glands of "two kidney, one clip" (2K1C) renovascular hypertension model in rats. The studies were carried out on the adrenal glands of rats after 3, 14, 28, 42, and 91 days from the renal artery clipping procedure. To identify neuroendocrine cells, immunohistochemical reaction was performed with the use of a specific antibody against CART. It was revealed that renovascular hypertension causes changes in the endocrine cells containing CART in the adrenal glands of rats. The changes observed in the endocrine cells depend on the time when the rats with experimentally induced hypertension were examined. In the first period of hypertension, the number and immunoreactivity of CART-containing cells were decreased, while from the 28-day test, it significantly increased, as compared to the control rats. CART is relevant to the regulation of homeostasis in the cardiovascular system and seems to be involved in renovascular hypertension. The results of the present work open the possibility of new therapeutic perspectives for the treatment of arterial hypertension, since CART function is involved in their pathophysiology.

• Authors: Irena Kasacka, Zaneta Piotrowska, Izabela Janiuk, Robert Zbucki
• Journal: Experimental biology and medicine (Maywood, N.J.) (Exp Biol Med (Maywood)) Vol. 239 Issue 10 Pg. 1292-9 (Oct 2014) ISSN: 1535-3699 [Electronic] England
• PMID: 24939825 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 by the Society for Experimental Biology and Medicine.

Chemical References

• Nerve Tissue Proteins
• Sympathomimetics
• Vasoconstrictor Agents
• cocaine- and amphetamine-regulated transcript protein
• Amphetamine
• Cocaine

Topics

• Adrenal Glands (drug effects, pathology)
• Amphetamine (administration & dosage)
• Animals
• Cocaine (administration & dosage)
• Disease Models, Animal
• Gene Expression Profiling
• Hypertension, Renovascular (pathology)
• Immunohistochemistry
• Male
• Nerve Tissue Proteins (biosynthesis, genetics)
• Rats, Wistar
• Sympathomimetics (administration & dosage)
• Time Factors
• Vasoconstrictor Agents (administration & dosage)