Rho kinase acts as a downstream molecule to participate in protein kinase Cε regulation of vascular reactivity after hemorrhagic shock in rats.

Abstract

Our previous study demonstrated that Rho kinase and protein kinase C (PKC) played important parts in the regulation of vascular reactivity after shock. Using superior mesenteric arteries (SMAs) from hemorrhagic shock rats and hypoxia-treated vascular smooth muscle cells (VSMCs), relationship of PKCε regulation of vascular reactivity to Rho kinase, as well as the signal transduction after shock, was investigated. The results showed that inhibition of Rho kinase with the Rho kinase-specific inhibitor Y-27632 antagonized the PKCε-specific agonist carbachol and highly expressed PKCε-induced increase of vascular reactivity in SMAs and VSMCs, whereas inhibition of PKCε with its specific inhibitory peptide did not antagonize the Rho kinase agonist (U-46619)-induced increase of vascular reactivity in SMAs and VSMCs. Activation of PKCε or highly expressed PKCε upregulated the activity of Rho kinase and the phosphorylation of PKC-dependent phosphatase inhibitor 17 (CPI-17), zipper interacting protein kinase (ZIPK), and integrin-linked kinase (ILK), whereas activation of Rho kinase increased only CPI-17 phosphorylation. The specific neutralization antibodies of ZIPK and ILK antagonized PKCε-induced increases in the activity of Rho kinase, but CPI-17 neutralization antibody did not antagonize this effect. These results suggested that Rho kinase takes part in the regulation of PKCε on vascular reactivity after shock. Rho kinase is downstream of PKCε. Protein kinase Cε activates Rho kinase via ZIPK and ILK; CPI-17 is downstream of Rho kinase.

• Authors: Tao Li, Yu Zhu, Jia-tao Zang, Xiao-yong Peng, Dan Lan, Guang-ming Yang, Jing Xu, Liang-ming Liu
• Journal: Shock (Augusta, Ga.) (Shock) Vol. 42 Issue 3 Pg. 239-45 (Sep 2014) ISSN: 1540-0514 [Electronic] United States
• PMID: 24827390 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Activators
• Muscle Proteins
• Phosphoproteins
• Ppp1r14a protein, rat
• Protein Kinase Inhibitors
• Vasoconstrictor Agents
• Prkce protein, rat
• integrin-linked kinase
• Dapk3 protein, rat
• Death-Associated Protein Kinases
• Protein Serine-Threonine Kinases
• rho-Associated Kinases
• Protein Kinase C-epsilon

Topics

• Animals
• Cells, Cultured
• Death-Associated Protein Kinases (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Enzyme Activation
• Enzyme Activators (pharmacology)
• Female
• In Vitro Techniques
• Male
• Mesenteric Artery, Superior (enzymology, physiopathology)
• Muscle Proteins (metabolism)
• Muscle, Smooth, Vascular (drug effects, enzymology, physiopathology)
• Myocytes, Smooth Muscle (drug effects, enzymology)
• Perfusion
• Phosphoproteins (metabolism)
• Phosphorylation
• Protein Kinase C-epsilon (antagonists & inhibitors, metabolism)
• Protein Kinase Inhibitors (pharmacology)
• Protein Serine-Threonine Kinases (metabolism)
• Rats, Sprague-Dawley
• Shock, Hemorrhagic (enzymology, physiopathology)
• Signal Transduction
• Vasoconstriction (drug effects)
• Vasoconstrictor Agents (pharmacology)
• rho-Associated Kinases (antagonists & inhibitors, metabolism)