The mitochondrial sulfur dioxygenase ETHYLMALONIC ENCEPHALOPATHY PROTEIN1 is required for amino acid catabolism during carbohydrate starvation and embryo development in Arabidopsis.

Abstract

The sulfur dioxygenase ETHYLMALONIC ENCEPHALOPATHY PROTEIN1 (ETHE1) catalyzes the oxidation of persulfides in the mitochondrial matrix and is essential for early embryo development in Arabidopsis (Arabidopsis thaliana). We investigated the biochemical and physiological functions of ETHE1 in plant metabolism using recombinant Arabidopsis ETHE1 and three transfer DNA insertion lines with 50% to 99% decreased sulfur dioxygenase activity. Our results identified a new mitochondrial pathway catalyzing the detoxification of reduced sulfur species derived from cysteine catabolism by oxidation to thiosulfate. Knockdown of the sulfur dioxygenase impaired embryo development and produced phenotypes of starvation-induced chlorosis during short-day growth conditions and extended darkness, indicating that ETHE1 has a key function in situations of high protein turnover, such as seed production and the use of amino acids as alternative respiratory substrates during carbohydrate starvation. The amino acid profile of mutant plants was similar to that caused by defects in the electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase complex and associated dehydrogenases. Thus, in addition to sulfur amino acid catabolism, ETHE1 also affects the oxidation of branched-chain amino acids and lysine.

• Authors: Lena Krüßel, Johannes Junemann, Markus Wirtz, Hannah Birke, Jeremy D Thornton, Luke W Browning, Gernot Poschet, Rüdiger Hell, Janneke Balk, Hans-Peter Braun, Tatjana M Hildebrandt
• Journal: Plant physiology (Plant Physiol) Vol. 165 Issue 1 Pg. 92-104 (May 2014) ISSN: 1532-2548 [Electronic] United States
• PMID: 24692429 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Arabidopsis Proteins
• DNA, Bacterial
• Sulfides
• Sulfites
• T-DNA
• Sulfur
• Dioxygenases
• ETHE1 protein, Arabidopsis
• sulfur dioxygenase
• Glutathione

Topics

• Amino Acids (metabolism)
• Arabidopsis (embryology, enzymology, growth & development)
• Arabidopsis Proteins (metabolism)
• Carbohydrate Metabolism
• DNA, Bacterial (genetics)
• Dioxygenases (metabolism)
• Gene Knockdown Techniques
• Glutathione (metabolism)
• Metabolic Networks and Pathways
• Mitochondria (enzymology)
• Models, Biological
• Mutagenesis, Insertional (genetics)
• Oxidation-Reduction
• Phenotype
• Seeds (embryology, enzymology)
• Substrate Specificity
• Sulfides (metabolism)
• Sulfites (metabolism)
• Sulfur (metabolism)