F2-isoprostanes as a biomarker of oxidative stress in the mouse bladder.

Abstract

PURPOSE:

We theorized that progressive bladder dysfunction due to clinical diagnoses such as outlet obstruction occurs as a result of cyclical oxidative stress events. We hypothesized that measurement of F2-isoprostane, a marker of lipid peroxidation, could serve as a biomarker of oxidative stress in the murine bladder.

MATERIALS AND METHODS:

At age 5 to 6 weeks oophorectomized female mice were subjected to 1 of 2 bladder injury models, that is partial bladder outlet obstruction or acute bladder distension. The time points studied after injury included 4, 8 and 16 weeks after obstruction, and 0 to 48 hours after acute bladder distension. In a separate group short-term repetitive acute bladder distension was performed every other day for 14 days. Bladder samples were analyzed for F2-isoprostane using gas chromatography and mass spectroscopy. Mean tissue F2-isoprostane levels were compared.

RESULTS:

F2-isoprostane increased significantly after 4 weeks of partial bladder outlet obstruction from 1.46 ng/gm in controls to 2.31 ng/gm at 4 weeks (p = 0.01). Eight and 16 weeks after partial bladder outlet obstruction F2-isoprostane remained significantly elevated (2.39 and 2.48 ng/gm, respectively). Acute bladder distension resulted in a significant increase in F2-isoprostane immediately after distension compared to controls (1.6 vs 0.75 ng/gm, p = 0.04). In mice that underwent repetitive acute bladder distension F2-isoprostane did not change.

CONCLUSIONS:

Measurement of tissue F2-isoprostane in the bladder reflects the progression of oxidative stress, primarily in chronic injury models such as partial bladder outlet obstruction. The usefulness of F2-isoprostane measurements in shorter term injury models requires further study.

• Authors: Douglass B Clayton, Heidi A Stephany, Christina B Ching, Shareena A Rahman, Stacy T Tanaka, John C Thomas, John C Pope 4th, Mark C Adams, John W Brock 3rd, Peter E Clark, Simon W Hayward, Robert J Matusik, Ginger L Milne
• Journal: The Journal of urology (J Urol) Vol. 191 Issue 5 Suppl Pg. 1597-601 (May 2014) ISSN: 1527-3792 [Electronic] United States
• PMID: 24679873 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• F2-Isoprostanes
• Reactive Oxygen Species

Topics

• Aging (physiology)
• Animals
• Biomarkers (blood)
• Disease Models, Animal
• Disease Progression
• F2-Isoprostanes (blood)
• Female
• Mice
• Oxidative Stress (physiology)
• Reactive Oxygen Species (blood)
• Urinary Bladder Neck Obstruction (blood, physiopathology)