Duodenal follicular lymphoma: comprehensive gene expression analysis with insights into pathogenesis.

Abstract

Follicular lymphoma (FL) of the gastrointestinal tract, particularly duodenal follicular lymphoma (DFL), is a rare variant of FL with indolent clinical behavior, and this disease is included in the 2008 World Health Organization classification system. In contrast to nodal follicular lymphoma (NFL), DFL occurs most frequently in the second part of the duodenum, lacks follicular dendritic cell meshworks and has memory B-cell characteristics. However, its molecular pathogenesis is still unclear. In the present study, we examined 10 DFL, 18 NFL and 10 gastric MALT lymphoma samples using gene expression analysis. Quantitative RT-PCR experiments and immunohistochemical analysis for 72 formalin-fixed, paraffin-embedded tissues from an independent series, including 32 DFL, 19 gastric MALT lymphoma and 27 NFL samples, were performed for validation of microarray data. Gene expression profiles of the three lymphoma types were compared using 2918 differentially expressed genes (DEG) and results suggested that DFL shares characteristics of MALT lymphoma. Among these DEG, CCL20 and MAdCAM-1 were upregulated in DFL and MALT but downregulated in NFL. In contrast, protocadherin gamma subfamily genes were upregulated in DFL and NFL. Quantitative RT-PCR and immunohistochemical studies demonstrated concordant results. Double immunofluorescence studies revealed that CCL20 and CCR6 were co-expressed in both DFL and MALT. We hypothesize that increased expression of CCL20 and MAdCAM-1 and co-expression of CCL20 and CCR6 may play an important role in tumorigenesis.

• Authors: Katsuyoshi Takata, Motohiko Tanino, Daisuke Ennishi, Akira Tari, Yasuharu Sato, Hiroyuki Okada, Yoshinobu Maeda, Naoe Goto, Hiroshi Araki, Mai Harada, Midori Ando, Masaya Iwamuro, Mitsune Tanimoto, Kazuhide Yamamoto, Randy D Gascoyne, Tadashi Yoshino
• Journal: Cancer science (Cancer Sci) Vol. 105 Issue 5 Pg. 608-15 (May 2014) ISSN: 1349-7006 [Electronic] England
• PMID: 24602001 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

Chemical References

• CCL20 protein, human
• CCR6 protein, human
• Cadherins
• Cell Adhesion Molecules
• Chemokine CCL20
• Immunoglobulins
• MADCAM1 protein, human
• Mucoproteins
• Receptors, CCR6

Topics

• Cadherins (biosynthesis, genetics)
• Cell Adhesion Molecules
• Cell Transformation, Neoplastic (genetics)
• Chemokine CCL20 (genetics)
• Dendritic Cells (immunology)
• Down-Regulation
• Duodenal Neoplasms (genetics, metabolism)
• Duodenum (pathology)
• Gene Expression Profiling
• Humans
• Immunoglobulins (genetics)
• Lymphoma, B-Cell, Marginal Zone (genetics, metabolism)
• Lymphoma, Follicular (genetics, metabolism)
• Molecular Sequence Data
• Mucoproteins (genetics)
• Oligonucleotide Array Sequence Analysis
• Receptors, CCR6 (biosynthesis, genetics)
• Up-Regulation