Differential genes in adipocytes induced from polycystic and non-polycystic ovary syndrome-derived human embryonic stem cells.

Abstract

We explored the molecular mechanisms of obesity and insulin resistance in patients with polycystic ovary syndrome (PCOS) using a human embryonic stem cell model (hESCs). Three PCOS-derived and one non-PCOS-derived hESC lines were induced into adipocytes, and then total RNA was extracted. The differentially expressed PCOS-derived and non-PCOS-derived adipocytes genes were identified using the Boao Biological human V 2.0 whole genome oligonucleotide microarray. Signals of interest were then validated by real-time PCR. A total of 153 differential genes were expressed of which 91 genes were up-regulated and 62 down-regulated. Nuclear receptor subfamily 0, group B, member 2 (NR0B2) was an up-regulated gene, and the GeneChip CapitalBio® Molecule Annotation System V4.0 indicated that it was associated with obesity and diabetes (Ratio ≥ 2.0X). Multiple genes are involved in PCOS. Nuclear receptor subfamily 0, group B, member 2 may play a role in obesity and insulin resistance in patients with PCOS.

• Authors: Fang Wang, Wei-Wei Liu, Xue-Mei Chen, Hui-Juan Kong, Jing Li, Ying-Pu Sun
• Journal: Systems biology in reproductive medicine (Syst Biol Reprod Med) Vol. 60 Issue 3 Pg. 136-42 (Jun 2014) ISSN: 1939-6376 [Electronic] England
• PMID: 24517280 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adipocytes (metabolism)
• Adipogenesis (genetics)
• Case-Control Studies
• Cell Line
• Embryonic Stem Cells (metabolism)
• Female
• Gene Expression Profiling (methods)
• Gene Expression Regulation
• Genetic Predisposition to Disease
• Humans
• Insulin Resistance (genetics)
• Obesity (genetics, metabolism, physiopathology)
• Oligonucleotide Array Sequence Analysis
• Phenotype
• Polycystic Ovary Syndrome (genetics, metabolism, physiopathology)
• Real-Time Polymerase Chain Reaction
• Reproducibility of Results