Brain angiogenesis inhibitor 1 is expressed by gastric phagocytes during infection with Helicobacter pylori and mediates the recognition and engulfment of human apoptotic gastric epithelial cells.

Abstract

After Helicobacter pylori infection in humans, gastric epithelial cells (GECs) undergo apoptosis due to stimulation by the bacteria or inflammatory cytokines. In this study, we assessed the expression and function of brain angiogenesis inhibitor 1 (BAI1) in the engulfment of apoptotic GECs using human tissue and cells. After induction of apoptosis by H. pylori or camptothecin, there was a 5-fold increase in the binding of apoptotic GECs to THP-1 cells or peripheral blood monocyte-derived macrophages as assayed by confocal microscopy or conventional and imaging flow cytometry. Binding was impaired 95% by pretreating apoptotic cells with annexin V, underscoring the requirement for phosphatidylserine recognition. The phosphatidylserine receptor BAI1 was expressed in human gastric biopsy specimens and gastric phagocytes. To confirm the role of BAI1 in apoptotic cell clearance, the functional domain of BAI1 was used as a competitive inhibitor or BAI1 expression was inhibited by small interfering RNA. Both approaches decreased binding and engulfment >40%. Exposing THP-1 cells to apoptotic cells inhibited IL-6 production from 1340 to <364 pg/ml; however, this decrease was independent of phagocytosis. We conclude that recognition of apoptotic cells by BAI1 contributes to their clearance in the human gastric mucosa and this is associated with anti-inflammatory effects.

• Authors: Soumita Das, Arup Sarkar, Kieran A Ryan, Sarah Fox, Alice H Berger, Ignacio J Juncadella, Diane Bimczok, Lesley E Smythies, Paul R Harris, Kodi S Ravichandran, Sheila E Crowe, Phillip D Smith, Peter B Ernst
• Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (FASEB J) Vol. 28 Issue 5 Pg. 2214-24 (May 2014) ISSN: 1530-6860 [Electronic] United States
• PMID: 24509909 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• ADGRB1 protein, human
• Angiogenic Proteins
• Cytokines
• Receptors, Cell Surface
• Receptors, G-Protein-Coupled
• phosphatidylserine receptor

Topics

• Angiogenic Proteins (metabolism)
• Apoptosis
• Cell Line
• Coculture Techniques
• Cytokines (metabolism)
• Epithelial Cells (metabolism, microbiology)
• Gastric Mucosa (cytology, microbiology)
• Gastritis (metabolism)
• Gene Expression Regulation
• Helicobacter Infections (metabolism)
• Helicobacter pylori
• Humans
• Inflammation
• Macrophages (cytology, metabolism)
• Monocytes (cytology)
• Phagocytes (cytology, metabolism)
• Phagocytosis
• Receptors, Cell Surface (chemistry)
• Receptors, G-Protein-Coupled
• Stomach (cytology, microbiology)