Abstract |
To study the anti- inflammation effect of Shikonin (Shik) and its mechanism, murine macrophage-like RAW264.7 cells (RAW264.7 cells) were divided into control group, LPS group (0.125, 0.25 and 0.5μg/ml), LPS (0.125, 0.25 and 0.5μg/ml) plus Shik (0.5, 1 and 2μM) group, and Shik (2μM) group. After exposure for 24h, the levels of Interleukin-6 (IL-6), nitric oxide (NO) and Tumor Necrosis Factor-α (TNF-α) in supernatant were measured with ELISA, the expression of high mobility group box 1( HMGB1) in supernatant and cytoplasm was assayed using qRT-PCR, western blot and immunofluorescence assays, the expression of IFN-β in cellular and supernatant was assayed by qRT-PCR and ELISA, and the ratio of nuclear to cytoplasm for NF-κB protein expression was assayed using western blot. The results of our investigation demonstrated that Shik could reduce significantly the levels of IL-6, NO and TNF-α in RAW264.7 cells exposed to LPS (P<0.05 or P<0.01). The expression of HMGB1, IFN-β and the ratio of nuclear to cytoplasm for NF-κB protein expression in LPS plus Shik group declined significantly as compared with LPS group (P<0.05 or P<0.01). The inhibitors of IFN-β signaling molecule JAK and NF-κB could attenuate significantly the expression of HMGB1 in supernatant. It was found in the present study that Shik could have the anti-inflammatory effects in RAW264.7 cells exposed to LPS, and one of the mechanisms may be the down-regulation of HMGB expression, which was associated with the IFN-β and NF-κB signaling pathways.
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Authors | Ying Yang, Jing Wang, Qiao Yang, Shanshan Wu, Zhenggang Yang, Haihong Zhu, Min Zheng, Weixia Liu, Wei Wu, Jiliang He, Zhi Chen |
Journal | International immunopharmacology
(Int Immunopharmacol)
Vol. 19
Issue 1
Pg. 81-7
(Mar 2014)
ISSN: 1878-1705 [Electronic] Netherlands |
PMID | 24447680
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- HMGB1 Protein
- HMGB1 protein, mouse
- Interleukin-6
- Lipopolysaccharides
- Naphthoquinones
- Transcription Factor RelA
- Tumor Necrosis Factor-alpha
- Nitric Oxide
- shikonin
- Interferon-beta
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Cell Line
- HMGB1 Protein
(antagonists & inhibitors, genetics, immunology)
- Interferon-beta
(genetics, immunology)
- Interleukin-6
(immunology)
- Lipopolysaccharides
- Mice
- Naphthoquinones
(pharmacology)
- Nitric Oxide
(immunology)
- Transcription Factor RelA
(immunology)
- Tumor Necrosis Factor-alpha
(immunology)
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