"One-stop shop" spectral imaging for rapid on-site diagnosis of lung cancer: a future concept in nano-oncology.

Abstract

BACKGROUND:

There are currently many techniques and devices available for the diagnosis of lung cancer. However, rapid on-site diagnosis is essential for early-stage lung cancer, and in the current work we investigated a new diagnostic illumination nanotechnology.

METHODS:

Tissue samples were obtained from lymph nodes, cancerous tissue, and abnormal intrapulmonary lesions at our interventional pulmonary suites. The following diagnostic techniques were used to obtain the samples: endobronchial ultrasound bronchoscopy; flexible bronchoscopy; and rigid bronchoscopy. Flexible and rigid forceps were used because several of the patients were intubated using a rigid bronchoscope. In total, 30 tissue specimens from 30 patients were prepared. CytoViva® illumination nanotechnology was subsequently applied to each of the biopsy tissue slides.

RESULTS:

A spectral library was created for adenocarcinoma, epidermal growth factor receptor mutation-positive adenocarcinoma, squamous cell carcinoma, usual interstitial pneumonitis, non-specific interstitial pneumonitis, typical carcinoid tumor, sarcoidosis, idiopathic pulmonary fibrosis, small cell neuroendocrine carcinoma, thymoma, epithelioid and sarcomatoid mesothelioma, cryptogenic organizing pneumonia, malt cell lymphoma, and Wegener's granulomatosis.

CONCLUSION:

The CytoViva software, once it had created a specific spectral library for each entity, was able to identify the same disease again in subsequent paired sets of slides of the same disease. Further evaluation of this technique could make this illumination nanotechnology an efficient rapid on-site diagnostic tool.

• Authors: Kaid Darwiche, Paul Zarogoulidis, Leslie Krauss, Filiz Oezkan, Robert Fred Henry Walter, Robert Werner, Dirk Theegarten, Leonidas Sakkas, Antonios Sakkas, Wolfgang Hohenforst-Scmidt, Konstantinos Zarogoulidis, Lutz Freitag
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 8 Pg. 4533-42 ( 2013) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 24285923 (Publication Type: Journal Article)

Topics

• Bronchoscopes
• Bronchoscopy
• Histocytochemistry
• Humans
• Lung Neoplasms (diagnosis, diagnostic imaging, pathology)
• Nanomedicine (methods)
• Spectrum Analysis (methods)
• Ultrasonography