Aqueous extract of Bambusae Caulis in Taeniam inhibits PMA-induced tumor cell invasion and pulmonary metastasis: suppression of NF-κB activation through ROS signaling.

Abstract

Bamboo shavings (Bambusae Caulis in Taeniam, BCT) are widely used as a traditional Chinese medicine to control hypertension and cardiovascular disease, and to alleviate fever, vomiting, and diarrhea. It has been demonstrated that BCT reduces ovalbumin-induced airway inflammation by regulating pro-inflammatory cytokines, and decreases tumor growth in tumor-bearing mice. However, the effects of BCT on the metastatic potential of malignant cancer cells and the detailed mechanism of its anti-metastatic activity have not been examined previously. In this study, we investigated whether an aqueous extract of BCT (AE-BCT) reduces the metastatic potential of HT1080 cells, and elucidated the underlying anti-metastatic mechanism. In addition, we examined whether AE-BCT administration inhibits pulmonary metastasis of intravenously injected B16F10 cells in C57BL/6J mice. AE-BCT (50-250 µg/ml) dose-dependently suppressed colony-forming activity under anchorage-dependent and -independent growth conditions. Pretreatment with AE-BCT efficiently inhibited cell migration, invasion, and adhesion. AE-BCT also dramatically suppressed PMA-induced MMP-9 activity and expression by blocking NF-κB activation and ERK phosphorylation. Production of intracellular ROS, a key regulator of NF-κB-induced MMP-9 activity, was almost completely blocked by pretreatment with AE-BCT. Furthermore, daily oral administration of AE-BCT at doses of 50 and 100 mg/kg efficiently inhibited lung metastasis of B16F10 cells injected into the tail veins of C57BL/6J mice with no systemic toxicity. These results demonstrate that AE-BCT significantly reduced the metastatic activity of highly malignant cancer cells by suppressing MMP-9 activity via inhibition of ROS-mediated NF-κB activation. These results indicate that AE-BCT may be a safe natural product for treatment of metastatic cancer.

• Authors: Aeyung Kim, Minju Im, Nam-Hui Yim, Young Pil Jung, Jin Yeul Ma
• Journal: PloS one (PLoS One) Vol. 8 Issue 10 Pg. e78061 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24205091 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Drugs, Chinese Herbal
• NF-kappa B
• Plant Extracts
• phorbolol myristate acetate
• Matrix Metalloproteinase 9
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Bambusa (chemistry)
• Blotting, Western
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Lung Neoplasms (metabolism, secondary)
• Matrix Metalloproteinase 9 (metabolism)
• Mice
• NF-kappa B (metabolism)
• Plant Extracts (chemistry, pharmacology)
• Signal Transduction (drug effects)
• Tetradecanoylphorbol Acetate (analogs & derivatives, toxicity)