Phosphorylation of Thr(1495) of nestin in a mouse model of cerebral ischemia and reperfusion damage.

Abstract

Nestin, a class VI intermediate filament protein, is expressed by neuronal progenitor cells in the subventricular zone (SVZ). In the present study, we analyzed the nestin expression and phosphorylation levels in nerve cells in a mouse model of cerebral ischemia and reperfusion. C57BL/6 mice were subjected to three-vessel occlusion for 14 min, and were killed either 1 or 4 days after the procedure. The percentages of cells in the SVZ that were positive for nestin, Thr(1495)-phosphorylated nestin or Ki67 did not significantly differ between the ischemic reperfusion and sham groups. Conversely, in the striatum and cornu ammonis 2 (CA2) regions, the mice at 4 days after ischemic reperfusion showed significantly higher numbers and percentages of nerve cells that were positive for nestin, Thr(1495)-phosphorylated nestin and Ki67 compared to results from the other groups. To our knowledge, this is the first description of phosphorylated nestin expression in neural progenitor cells in the SVZ of adult mice. In this cerebral ischemia and reperfusion mouse model, cells positive for Thr(1495)-phosphorylated nestin were increased in the striatum and CA2 field of the hippocampus; suggesting that nestin phosphorylation may play an important role in mitotically active neuronal progenitor cells.

• Authors: Yoko Matsuda, Go Suzuki, Teruo Kusano, Yoko Kawamoto, Hisashi Yoshimura, Akira Fuse, Hiroyuki Yokota, Zenya Naito, Toshiyuki Ishiwata
• Journal: Pathology international (Pathol Int) Vol. 63 Issue 9 Pg. 448-56 (Sep 2013) ISSN: 1440-1827 [Electronic] Australia
• PMID: 24200156 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 The Authors. Pathology International © 2013 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

Chemical References

• Nestin
• Threonine
• Phosphotransferases

Topics

• Animals
• Brain Ischemia (metabolism)
• Disease Models, Animal
• Male
• Mice
• Mice, Inbred C57BL
• Molecular Sequence Data
• Nestin (genetics, metabolism)
• Phosphorylation (physiology)
• Phosphotransferases (metabolism)
• Reperfusion Injury (metabolism)
• Stem Cells (metabolism)
• Threonine (metabolism)