Abstract | BACKGROUND: Previous studies suggest that dendritic cells and macrophages play an important role in inflammation of eosinophilic pneumonia. The mechanism of dendritic cell and macrophage accumulation into the lung, however, is unknown. Here, we hypothesized that CCR7 ligands, CCL19 and CCL21, contribute to the accumulation of dendritic cells and alveolar macrophages in the inflamed lung of patients with eosinophilic pneumonia. METHODS: RESULTS: CCL19 and CCL21 levels in the bronchoalveolar lavage fluid were significantly higher in patients with eosinophilic pneumonia than in controls. Levels of CCL19, but not CCL21, were statistically correlated with the levels of CCL16, CCL17 and CCL22 in patients with eosinophilic pneumonia. Immunocytochemistry revealed CCL19 expression in dendritic cells, macrophages and T-lymphocytes harvested from patients with eosinophilic pneumonia, and CCR7 expression in dendritic cells and macrophages. Levels of CCL19, but not CCL21, were significantly decreased after remission in patients with eosinophilic pneumonia. After provocation tests, CCL19 levels were elevated in all patients with eosinophilic pneumonia. CONCLUSIONS: These findings indicate that CCL19 rather than CCL21 may contribute to the accumulation of dendritic cells and macrophages in the inflamed lungs of patients with eosinophilic pneumonia.
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Authors | S Nureki, E Miyazaki, T Ishi, T Ito, R Takenaka, M Ando, T Kumamoto |
Journal | Allergy
(Allergy)
Vol. 68
Issue 11
Pg. 1387-95
(Nov 2013)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 24111618
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- CCL19 protein, human
- CCL21 protein, human
- CCR7 protein, human
- Chemokine CCL19
- Chemokine CCL21
- Ligands
- Receptors, CCR7
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Bronchoalveolar Lavage Fluid
(immunology)
- Chemokine CCL19
(biosynthesis, metabolism)
- Chemokine CCL21
(biosynthesis, metabolism)
- Chronic Disease
- Dendritic Cells
(metabolism, pathology)
- Female
- Humans
- Inflammation
(chemically induced, immunology, pathology)
- Ligands
- Macrophages, Alveolar
(metabolism, pathology)
- Male
- Middle Aged
- Pulmonary Eosinophilia
(chemically induced, immunology, pathology)
- Receptors, CCR7
(biosynthesis, metabolism)
- Up-Regulation
(immunology)
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