Abstract | BACKGROUND: METHODS: Using primary enteric neurons and colon explant cultures from wild type and NPY knockout (NPY(-/-)) mice, we determined if NPY knockdown modulates TNF release and epithelial permeability. Further, we assessed if NPY expression is inducible by TNF in enteric neuronal cells and mouse model of experimental colitis, using the TNF inhibitors- etanercept (blocks transmembrane and soluble TNF) and XPro1595 (blocks soluble TNF only). RESULTS: We found that enteric neurons express TNF receptors ( TNFR1 and R2). Primary enteric neurons from NPY(-/-) mice produced less TNF compared with wild type. Further, TNF activated NPY promoter in enteric neurons through phospho-c-Jun. NPY(-/-) mice had decreased intestinal permeability. In vitro, NPY increased epithelial permeability through phosphatidyl inositol-3-kinase (PI3-K)-induced pore-forming claudin-2. TNF inhibitors attenuated NPY expression in vitro and in vivo. TNF inhibitor-treated colitic mice exhibited reduced NPY expression and inflammation, reduced oxidative stress, enhanced neuronal survival, and improved colonic motility. XPro1595 had more protective effects on neuronal survival and motility compared with etanercept. CONCLUSIONS: We demonstrate a novel TNF-NPY cross talk that modulates inflammation, barrier functions, and colonic motility during inflammation. It is also suggested that selective blocking of soluble TNF may be a better therapeutic option than using anti-TNF antibodies.
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Authors | Bindu Chandrasekharan, Sabrina Jeppsson, Stefan Pienkowski, Denise D Belsham, Shanthi V Sitaraman, Didier Merlin, Efi Kokkotou, Asma Nusrat, Malu G Tansey, Shanthi Srinivasan |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 19
Issue 12
Pg. 2535-46
(Nov 2013)
ISSN: 1536-4844 [Electronic] England |
PMID | 24108115
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Neuropeptide Y
- Tumor Necrosis Factor-alpha
- Phosphatidylinositol 3-Kinases
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Topics |
- Animals
- Blotting, Western
- Case-Control Studies
- Cell Membrane Permeability
- Chromatin Immunoprecipitation
- Colitis
(metabolism, pathology)
- Colon
(physiology)
- Cytokines
(metabolism)
- Disease Models, Animal
- Electric Conductivity
- Enteric Nervous System
(metabolism, pathology)
- Gastrointestinal Motility
(physiology)
- Humans
- Intestinal Mucosa
(metabolism, pathology)
- Laser Capture Microdissection
- Mice
- Mice, Knockout
- Mutagenesis, Site-Directed
- Neuropeptide Y
(antagonists & inhibitors, metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Signal Transduction
(physiology)
- Tumor Necrosis Factor-alpha
(antagonists & inhibitors, metabolism)
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