Increased levels of plasma
homocysteine (
hyperhomocysteinemia-HHcy) are associated to the development of
coronary artery disease (CAD),
peripheral vascular disease and
thrombosis. In addition, recent studies have shown that
inflammation, probably mediated by macrophages, mediates the pathogenesis associated to high levels of
homocysteine (Hcy). In the present study, we evaluated the Hcy effects in the
ATP hydrolysis and its breakdown products in murine macrophages. The results showed that micromolar concentrations of Hcy increased the
ATP,
ADP and
AMP hydrolysis. Additionally, our results show decreased
inosine levels in the extracellular milieu of Hcy-exposed macrophages. The increasing in
ATP,
ADP and
AMP hydrolysis are not explained by increased transcription or
protein expression of NTPDases and
ecto-5'-nucleotidase (ecto-5'-NT/CD73)
enzymes. Moreover, the formation of
reactive oxygen species did not interfere in the Hcy effects, which suggest that Hcy or Hcy metabolites act directly on the modulation of NTPDases and ecto-5'-NT/CD73 activities. In conclusion, Hcy induces the rapid breakdown of
ATP,
ADP and
AMP to
adenosine (
ADO), which is classically known as an anti-inflammatory response in immune cells. However, by the action of these
enzymes, the extracellular
adenosine generated during Hcy treatment probably is uptaken into the cells, as evidenced by the decreased in
inosine formation, and thus collaborating to the inflammatory complications associates to HHcy.