[Changes of transcription factors Bcl-6, Foxp3 and RORγt in CD4(+) cells in bone marrow of immuno-related hematocytopenia].

Abstract

OBJECTIVE:

To evaluate the possible mechanism of transcription factors B cell lymphoma 6 (Bcl-6) , forkhead/winged helix transcription factor 3 (Foxp3) and retinoic acid related orphan receptor (RORγt) in CD4(+) T cells for immuno-related hematocytopenia (IRH).

METHODS:

CD4(+) T cells were harvested from 40 IRH patients, 38 aplastic anemia subjects and 25 normal controls and separated by magnetic activated cell sorting (MACS). Then the expressions of transcription factors of Foxp3, RORγ and Bcl-6 in CD4(+) T cells were measured by real time fluorescent quantitative-polymerase chain reaction (QRT-PCR).

RESULTS:

Auto-antibody was detected on CD34(+) cells (67.5% (27/40) ), CD15(+) cells (65.0% (26/40)), GlyA(+) cells (75.0% (30/40) ), auto-antibody involving three, two or one myeloid cell were detected in 27.5% (11/40), 52.5% (21/40), 20.0% (8/40) of IRH patients. Compensatory increase of Foxp3 mRNA was found in IRH (0.124 (0.073-0.198) vs 0.071 (0.046-0.118), P < 0.05). The expression of Bcl-6 was higher (2.243 (0.854-4.544) vs 1.211 (0.131-2.816), P < 0.05). Compared to aplastic anemia, the expression of RORγt was lower in IRH (0.133 (0.068-0.189) vs 0.290 (0.138-0.480), P < 0.01) and the ratio of Treg/Th17 shifted to Th17 in patients with aplastic anemia (Foxp3/RORγt ratio,0.500 (0.240-0.795) vs 0.975 (0.483-1.416), P < 0.01).

CONCLUSION:

As one kind of bone marrow failures caused by autoantibody to bone marrow cells, IRH may occur due to a high expression of Bcl-6 in CD4(+) T cells, its immunopathogenesis is different from that of aplastic anemia.

• Authors: Bao-zhi Fang, Jian-nong Cen, Ming-qing Zhu, Guang-sheng He, Miao Miao, Xiu-li Wang, Qian Wu, Zhen-zhen Liu, Cong Lu, Ying-xin Sun, De-pei Wu, Ai-ning Sun, Chang-geng Ruan
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 93 Issue 20 Pg. 1529-32 (May 28 2013) ISSN: 0376-2491 [Print] China
• PMID: 24028716 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BCL6 protein, human
• DNA-Binding Proteins
• FOXP3 protein, human
• Forkhead Transcription Factors
• Nuclear Receptor Subfamily 1, Group F, Member 3
• Proto-Oncogene Proteins c-bcl-6
• RNA

Topics

• Adolescent
• Adult
• Aged
• Bone Marrow (metabolism)
• CD4-Positive T-Lymphocytes (metabolism)
• Case-Control Studies
• Child
• Child, Preschool
• DNA-Binding Proteins (metabolism)
• Female
• Forkhead Transcription Factors (metabolism)
• Humans
• Male
• Middle Aged
• Nuclear Receptor Subfamily 1, Group F, Member 3 (metabolism)
• Pancytopenia (etiology, immunology, metabolism)
• Proto-Oncogene Proteins c-bcl-6
• RNA (genetics)
• Young Adult