Association of interferon-gamma induced protein 10 promoter polymorphisms with the disease progression of hepatitis B virus infection in Chinese Han population.

Abstract

BACKGROUND AND AIMS:

Interferon-gamma induced protein 10 (IP-10) was suggested to be involved in liver injury in viral hepatitis. This study aimed to investigate the impact of the single nucleotide polymorphisms (SNP) G-201A (rs1439490) in IP-10 gene on disease progression of hepatitis B virus (HBV) infection.

METHODS:

The -201 SNP in IP-10 promoter was genotyped from 577 patients with different illness categories and 275 health controls; In vitro IP-10 promoter activity was compared between haplotype GG and AA homozygotes using luciferase reporter system in HepG2 cells. In vivo expression of IP-10 was compared between patients with -201 AA genotype and GG genotype.

RESULTS:

The detected frequency of G-201A SNP was 17.8%, 25.3%, 26.6%, and 13.8% for patients with acute hepatitis B (AHB), patients with mild chronic hepatitis B (CHB-M), patients with severe chronic hepatitis B (CHB-S), and health controls, respectively. In vitro IP-10 promoter-driven luciferase activity in pGL3-Enhancer-201A transfected HepG2 cells was 1.43-fold higher than that in pGL3-Enhancer-201G transfected HepG2 cells (P<0.01). In vivo IP-10 transcriptional expression of peripheral blood mononuclear cells was 1.38-fold higher in patients with -201 AA genotype than in patients with -201 GG genotype (P<0.01).

CONCLUSION:

G-201A in promoter region of IP-10 gene was associated with liver disease progression in patients with HBV infection through up-regulating IP-10 expression.

• Authors: Zhihui Xu, Yan Liu, Liming Liu, Xiaodong Li, Siyu Bai, Yihui Rong, Haibin Wang, Yuanli Mao, Shaojie Xin, Dongping Xu
• Journal: PloS one (PLoS One) Vol. 8 Issue 9 Pg. e72799 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24023775 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CXCL10 protein, human
• Chemokine CXCL10

Topics

• Adult
• Asian People (genetics)
• Chemokine CXCL10 (genetics)
• Female
• Genetic Predisposition to Disease
• Hepatitis B (genetics)
• Humans
• Male
• Polymorphism, Genetic (genetics)
• Young Adult