Abstract | OBJECTIVE: METHODS: RESULTS:
Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50 = 12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer.
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Authors | Farha A Kabeer, Geetha B Sreedevi, Mangalam S Nair, Dhanya S Rajalekshmi, Latha P Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan |
Journal | Journal of integrative medicine
(J Integr Med)
Vol. 11
Issue 4
Pg. 269-77
(Jul 2013)
ISSN: 2095-4964 [Print] Netherlands |
PMID | 23867245
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Lactones
- Sesquiterpenes
- deoxyelephantopin
- Caspases
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Topics |
- Adenocarcinoma
(drug therapy, pathology)
- Adenocarcinoma of Lung
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Apoptosis
(drug effects)
- Caspases
(physiology)
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Dose-Response Relationship, Drug
- Humans
- Lactones
(pharmacology)
- Lung Neoplasms
(drug therapy, pathology)
- Sesquiterpenes
(pharmacology)
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