Systemic delivery of small interfering RNA by use of targeted polycation liposomes for cancer therapy.

Abstract

Novel polycation liposomes decorated with cyclic(Cys-Arg-Gly-Asp-D-Phe) peptide (cyclicRGD)-polyethylene glycol (PEG) (RGD-PEG-polycation liposomes (PCL)) were previously developed for cancer therapy based on RNA interference. Here, we demonstrate the in vivo delivery of small interfering RNA (siRNA) to tumors by use of RGD-PEG-PCL in B16F10 melanoma-bearing mice. Pharmacokinetic data obtained by positron emission tomography showed that cholesterol-conjugated siRNA formulated in RGD-PEG-PCL markedly accumulated in the tumors. Delivered by RGD-PEG-PCL, a therapeutic cocktail of siRNAs composed of cholesterol-conjugated siRNAs for c-myc, MDM2, and vascular endothelial growth factor (VEGF) were able to significantly inhibit the growth of B16F10 melanoma both in vitro and in vivo. These data suggest that targeted delivery of siRNAs by use of RGD-PEG-PCL has considerable potential for cancer treatment.

• Authors: Eriya Kenjo, Tomohiro Asai, Norihito Yonenaga, Hidenori Ando, Takayuki Ishii, Kentaro Hatanaka, Kosuke Shimizu, Yugo Urita, Takehisa Dewa, Mamoru Nango, Hideo Tsukada, Naoto Oku
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 36 Issue 2 Pg. 287-91 ( 2013) ISSN: 1347-5215 [Electronic] Japan
• PMID: 23370357 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ethylenediamines
• Liposomes
• Organophosphates
• Peptides, Cyclic
• RNA, Small Interfering
• Vascular Endothelial Growth Factor A
• cyclic arginine-glycine-aspartic acid peptide
• vascular endothelial growth factor A, mouse
• dicetylphosphate
• Polyethylene Glycols
• Mdm2 protein, mouse
• Proto-Oncogene Proteins c-mdm2
• Amy1 protein, mouse
• Salivary alpha-Amylases
• tetraethylenepentamine

Topics

• Animals
• Cell Proliferation (drug effects)
• Ethylenediamines (chemistry)
• Liposomes
• Lung (metabolism)
• Lung Neoplasms (therapy)
• Male
• Melanoma, Experimental (therapy)
• Mice
• Mice, Inbred C57BL
• Organophosphates (chemistry)
• Peptides, Cyclic (chemistry)
• Polyethylene Glycols (chemistry)
• Proto-Oncogene Proteins c-mdm2 (genetics)
• RNA, Small Interfering (administration & dosage)
• Salivary alpha-Amylases (genetics)
• Vascular Endothelial Growth Factor A (genetics)