Quercetin decreases liver damage in mice with non-alcoholic steatohepatitis.

Abstract

Non-alcoholic steatohepatitis (NASH) is a frequent condition in obese patients that may progress to end-stage liver disease. This study was designed to evaluate the modulation of this condition by use of quercetin (Q), a flavonoid largely found in vegetable foods, with known anti-inflammatory and antioxidant properties, in the experimental model of non-alcoholic steatohepatitis (NASH) using a diet deficient in methionine and choline (MCD). Male C57BL6 mice were divided into four groups (n = 16): (i) Control plus vehicle (control ration plus carboxymethylcellulose 1% used as vehicle, CO + V); (ii) Control ration plus Q 50 mg/kg (CO + Q); (iii) MCD diet plus vehicle (NASH + V); and (iv) MCD diet plus Q (NASH + Q). Diets were administered for 4 weeks. At the end of the experimental period, liver alterations, bioindicators of oxidative stress and DNA damage were assessed. NASH was diagnosed in 100% of the mice that were fed the MCD diet. In addition, a significant increase in DNA damage in liver tissue from NASH + V group was observed in comparison with CO + V. The group NASH + Q showed a significant decrease in hepatic damage enzymes, lipoperoxidation, DNA damage and a lower degree of macrovesicular steatosis, ballooning and inflammatory process. These findings suggest that Q may have protective effects by improving liver integrity in NASH.

• Authors: Eder Marcolin, Luiz Felipe Forgiarini, Graziella Rodrigues, Juliana Tieppo, Greice Stefani Borghetti, Valquiria Linck Bassani, Jaqueline Nascimento Picada, Norma Possa Marroni
• Journal: Basic & clinical pharmacology & toxicology (Basic Clin Pharmacol Toxicol) Vol. 112 Issue 6 Pg. 385-91 (Jun 2013) ISSN: 1742-7843 [Electronic] England
• PMID: 23331460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)
• Copyright: © 2013 Nordic Pharmacological Society. Published by John Wiley & Sons Ltd.

Chemical References

• Antioxidants
• Quercetin
• Methionine

Topics

• Animals
• Antioxidants (administration & dosage, therapeutic use)
• Choline Deficiency
• Comet Assay
• DNA Damage (drug effects)
• Diet
• Disease Models, Animal
• Fatty Liver (etiology, metabolism, pathology, prevention & control)
• Lipid Peroxidation (drug effects)
• Liver Function Tests
• Male
• Methionine (deficiency)
• Mice
• Mice, Inbred C57BL
• Molecular Structure
• Oxidative Stress (drug effects)
• Quercetin (administration & dosage, therapeutic use)