Murine colitis is mediated by vimentin.

Abstract

Vimentin, an abundant intermediate filament protein, presumably has an important role in stabilizing intracellular architecture, but its function is otherwise poorly understood. In a vimentin knockout (Vim KO) mouse model, we note that Vim KO mice challenged with intraperitoneal Escherichia coli control bacterial infection better than do wild-type (WT) mice. In vitro, Vim KO phagocytes show significantly increased capacity to mediate bacterial killing by abundant production of reactive oxygen species (ROS) and nitric oxides, likely due to interactions with the p47phox active subunit of NADPH oxidase. In acute colitis induced by dextran sodium sulfate (DSS), Vim KO mice develop significantly less gut inflammation than do WT mice. Further, Vim KO mice have markedly decreased bacterial extravasation in the setting of DSS-induced acute colitis, consistent with decreased intestinal disease. Our results suggest that vimentin impedes bacterial killing and production of ROS, thereby contributing to the pathogenesis of acute colitis.

• Authors: Nirit Mor-Vaknin, Maureen Legendre, Yue Yu, Carlos H C Serezani, Sanjay K Garg, Anna Jatzek, Michael D Swanson, Marta J Gonzalez-Hernandez, Seagal Teitz-Tennenbaum, Antonello Punturieri, N Cary Engleberg, Ruma Banerjee, Marc Peters-Golden, John Y Kao, David M Markovitz
• Journal: Scientific reports (Sci Rep) Vol. 3 Pg. 1045 ( 2013) ISSN: 2045-2322 [Electronic] England
• PMID: 23304436 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• RNA, Small Interfering
• Reactive Oxygen Species
• Vimentin
• Nitric Oxide
• Dextran Sulfate
• NADPH Oxidases
• neutrophil cytosolic factor 1

Topics

• Animals
• Colitis (chemically induced, metabolism, pathology)
• Dextran Sulfate (toxicity)
• Escherichia coli (pathogenicity)
• Macrophages (immunology, metabolism)
• Mice
• Mice, Knockout
• NADPH Oxidases (metabolism)
• Nitric Oxide (metabolism)
• Phagocytosis
• RNA Interference
• RNA, Small Interfering (metabolism)
• Reactive Oxygen Species (metabolism)
• Vimentin (antagonists & inhibitors, genetics, metabolism)