A peptide vaccine based on a B-cell epitope on the VP1 protein of enterovirus 70 induces a strong antibody response.

Abstract

Enterovirus 70 (EV70) is the causative agent of acute hemorrhagic conjunctivitis (AHC), for which no effective vaccine is available. This study revealed a high reactivity of the N-terminal region of EV70 VP1 (VP1-1) with an anti-EV70 mouse serum. The analysis of overlapping synthetic peptides of VP1-1 identified a B-cell epitope in this region. The E-peptide (14-ANTVESEIKAELGVI-28) showing the highest reactivity with the anti-EV70 serum induced neutralizing antibodies in mice and reduced the virus titer in the eyes, suggesting that it is a candidate vaccine against AHC caused by EV70.

• Authors: Kee-Bum Park, Byung-Kwan Lim, Michael B Ye, Soo-Young Chung, Jae-Hwan Nam
• Journal: Acta virologica (Acta Virol) Vol. 56 Issue 4 Pg. 337-42 ( 2012) ISSN: 0001-723X [Print] Slovakia
• PMID: 23237090 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Capsid Proteins
• Epitopes, B-Lymphocyte
• Peptides
• Vaccines, Subunit

Topics

• Animals
• Antibodies, Viral (immunology)
• Antibody Formation
• Capsid Proteins (genetics, immunology)
• Conjunctivitis, Acute Hemorrhagic (immunology, prevention & control, virology)
• Enterovirus D, Human (genetics, immunology)
• Epitopes, B-Lymphocyte (immunology)
• Humans
• Mice
• Mice, Inbred BALB C
• Peptides (genetics, immunology)
• Vaccines, Subunit (immunology)