Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases.

Abstract

Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

• Authors: Qiang Wang, Leiyun Weng, Hongbing Jiang, Shijian Zhang, Tetsuya Toyoda
• Journal: Analytical biochemistry (Anal Biochem) Vol. 433 Issue 2 Pg. 92-4 (Feb 15 2013) ISSN: 1096-0309 [Electronic] United States
• PMID: 23103398 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• DNA Primers
• Viral Proteins
• RNA-Dependent RNA Polymerase

Topics

• Cell-Free System
• DNA Primers (chemistry)
• Fluorescence
• Humans
• RNA Viruses (enzymology)
• RNA-Dependent RNA Polymerase (chemistry)
• Transcription, Genetic
• Viral Proteins (chemistry)