Abstract | AIM: METHODS: AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats. Sera and pancreata were assayed at 24 h for the parameters listed above; we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA. RESULTS: At 24 h after induction of AP, the pancreata of older animals had less edema (mean ± SE histologic score of young vs old: 3.11 ± 0.16 vs 2.50 ± -0.11, P < 0.05), decreased local inflammatory response (histologic score of stromal infiltrate: 3.11 ± 0.27 vs 2.00 ± 0.17, P < 0.05) and increased bacterial infiltration (174% ± 52% increase from sham vs 377% ± 4%, P < 0.05). A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining. There were no differences in serum amylase and lipase activity, or tissue myeloperoxidase or monocyte chemotactic protein-1 levels. However, in the most-aged group, serum C-reactive protein levels were higher (young vs old: 0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL, P < 0.05). CONCLUSION: In older animals, there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level; this may explain the more aggressive clinical course.
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Authors | Sophia Fu, Albert Stanek, Cathy M Mueller, Nefertti A Brown, Chongmin Huan, Martin H Bluth, Michael E Zenilman |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 18
Issue 26
Pg. 3379-88
(Jul 14 2012)
ISSN: 2219-2840 [Electronic] United States |
PMID | 22807607
(Publication Type: Journal Article)
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Chemical References |
- Antigens, Neoplasm
- Biomarkers, Tumor
- Lectins, C-Type
- Pancreatitis-Associated Proteins
- REG3A protein, human
- RNA, Ribosomal
- Reg3a protein, rat
- Reg3b protein, rat
- Taurocholic Acid
- C-Reactive Protein
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Topics |
- Acute Disease
- Aging
- Animals
- Antigens, Neoplasm
(biosynthesis)
- Biomarkers, Tumor
(biosynthesis)
- C-Reactive Protein
(metabolism)
- Genomics
- Immunohistochemistry
(methods)
- Inflammation
- Lectins, C-Type
(biosynthesis)
- Male
- Pancreatitis
(diagnosis, metabolism)
- Pancreatitis-Associated Proteins
- RNA, Ribosomal
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Ribosomes
(metabolism)
- Taurocholic Acid
(pharmacology)
- Time Factors
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