Effects of PEMF and glucocorticoids on proliferation and differentiation of osteoblasts.

Abstract

Pulsed electromagnetic fields (PEMF) can promote bone healing, while use of dexamethasone induces bone loss and osteoporosis. There is no report available on the combined effects of PEMF and dexamethasone on the activity of osteoblasts. Here, we investigated the effects of PEMF and dexamethasone on the proliferation and differentiation of MC3T3-E1 osteoblasts. Our results showed that PEMF and dexamethasone respectively increased and decreased the proliferation of MC3T3-E1 osteoblasts, meanwhile PEMF eliminated the effect of dexamethasone on MC3T3-E1 osteoblasts. Moreover, we also found that dexamethasone combined with PEMF upregulated the mRNA expression of IGF-1 at the early stage after the stimulation of PEMF and improved the decrease of COX-2 mRNA expression induced by dexamethasone at the late stage after the stimulation of PEMF. PEMF may be beneficial to improve dexamethasone-induced bone loss and osteoporosis.

• Authors: Mohammed Y Esmail, Lijun Sun, Liyin Yu, Hao Xu, Liang Shi, Jianbao Zhang
• Journal: Electromagnetic biology and medicine (Electromagn Biol Med) Vol. 31 Issue 4 Pg. 375-81 (Dec 2012) ISSN: 1536-8386 [Electronic] England
• PMID: 22676065 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glucocorticoids
• RNA, Messenger
• Dexamethasone

Topics

• Animals
• Cell Differentiation (drug effects, radiation effects)
• Cell Line
• Cell Proliferation (drug effects, radiation effects)
• Dexamethasone (pharmacology)
• Electromagnetic Fields
• Gene Expression Regulation, Enzymologic (drug effects, radiation effects)
• Glucocorticoids (pharmacology)
• Mice
• Osteoblasts (cytology, drug effects, metabolism, radiation effects)
• RNA, Messenger (genetics, metabolism)
• Time Factors