Abstract | OBJECTIVES: METHODS:
COL1A2 expression was examined in a panel of cell lines using RT-PCR. The methylation status of the COL1A2 promoter was studied using bisulfate sequencing and methylation-specific PCR (MSP). RESULTS:
COL1A2 expression was absent in 6 of 11 (54.5%) UM-SCC cell lines, whereas three nonmalignant cell lines had stable expressions. MSP analysis showed that 46/98 (46.9%) contained methylated alleles. COL1A2 methylation was significantly correlated with tumor size (P = 0.041), lymph node status (P = 0.008), tumor stage (P = 0.011), H-cadherin methylation (P = 0.039) and disease-free survival (P = 0.005). On multivariate Cox proportional hazard regression, which included age, sex, smoking status, and alcohol exposure, both tumor stage and COL1A2 methylation remained independent prognostic factors. CONCLUSIONS: This study suggests that CpG hypermethylation is a likely mechanism of COL1A2 gene inactivation, supporting the hypothesis that the COL1A2 gene may play a role in the tumorigenesis of HNSCC and may serve as an important biomarker.
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Authors | Kiyoshi Misawa, Takeharu Kanazawa, Yuki Misawa, Atsushi Imai, Shiori Endo, Katsura Hakamada, Hiroyuki Mineta |
Journal | Cancer biomarkers : section A of Disease markers
(Cancer Biomark)
2011-2012
Vol. 10
Issue 3-4
Pg. 135-44
ISSN: 1875-8592 [Electronic] Netherlands |
PMID | 22674299
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Cadherins
- Collagen Type I
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Topics |
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(genetics)
- Cadherins
(genetics)
- Carcinoma, Squamous Cell
(genetics, pathology, therapy)
- Cell Line
- Cell Line, Tumor
- Collagen Type I
(genetics)
- CpG Islands
(genetics)
- DNA Methylation
- Disease-Free Survival
- Female
- Gene Expression Regulation, Neoplastic
- Head and Neck Neoplasms
(genetics, pathology, therapy)
- Humans
- Lymphatic Metastasis
- Male
- Middle Aged
- Multivariate Analysis
- Neoplasm Staging
- Outcome Assessment, Health Care
(statistics & numerical data)
- Predictive Value of Tests
- Prognosis
- Proportional Hazards Models
- Reverse Transcriptase Polymerase Chain Reaction
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