[Effects of AZT on leukemia cell line KG-1a proliferation and telomerase activity].

Abstract

This study was purposed to investigate the effect of 3'-azido-2', 3'-dideoxythymidine (AZT)on the proliferation and telomerase activity of human acute myeloid leukemia cell line KG-1a. The effect of proliferation was detected by MTT assay after the KG-1a cell were stimulated for 24, 48 and 72 h with different concentrations of AZT; telomerase activity was detected with TRAP-PCR-ELISA assay; RT-PCR was used to detect telomerase hTERT mRNA expression. The results showed that the proliferation of KG-1a cells was inhibited in a time and concentration dependent manner after exposure to AZT for 24, 48 and 72 h; the KG-1a cells decreased in S phase and increased in G(2)/M phase with the increasing of the concentration of AZT; telomerase activity and hTERT-mRNA expression in the experimental groups decreased after treated with AZT, which was positively correlated with concentration of AZT. It is concluded that AZT inhibits KG-1a cell proliferation and induces apoptosis, which maybe related with its decreasing the telomerase activity and hTERT mRNA expression.

• Authors: Rui-Rui Jin, Rong Chao, Ya-Ming Xi, Che Chen, Hui-Yuan Chu, Ming Li, Hao Zhang
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 20 Issue 2 Pg. 277-81 (Apr 2012) ISSN: 1009-2137 [Print] China
• PMID: 22541081 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Zidovudine
• Telomerase

Topics

• Apoptosis (drug effects)
• Cell Cycle
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Humans
• Leukemia (metabolism, pathology)
• Telomerase (antagonists & inhibitors, metabolism)
• Zidovudine (pharmacology)