Protection against lethal leptospirosis after vaccination with LipL32 coupled or coadministered with the B subunit of Escherichia coli heat-labile enterotoxin.

Abstract

Leptospirosis, a worldwide zoonosis, lacks an effective, safe, and cross-protective vaccine. LipL32, the most abundant, immunogenic, and conserved surface lipoprotein present in all pathogenic species of Leptospira, is a promising antigen candidate for a recombinant vaccine. However, several studies have reported a lack of protection when this protein is used as a subunit vaccine. In an attempt to enhance the immune response, we used LipL32 coupled to or coadministered with the B subunit of the Escherichia coli heat-labile enterotoxin (LTB) in a hamster model of leptospirosis. After homologous challenge with 5× the 50% lethal dose (LD(50)) of Leptospira interrogans, animals vaccinated with LipL32 coadministered with LTB and LTB::LipL32 had significantly higher survival rates (P < 0.05) than animals from the control group. This is the first report of a protective immune response afforded by a subunit vaccine using LipL32 and represents an important contribution toward the development of improved leptospirosis vaccines.

• Authors: André A Grassmann, Samuel R Félix, Carolina Ximendes dos Santos, Marta G Amaral, Amilton C P Seixas Neto, Michel Q Fagundes, Fabiana K Seixas, Everton F da Silva, Fabricio R Conceição, Odir A Dellagostin
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 19 Issue 5 Pg. 740-5 (May 2012) ISSN: 1556-679X [Electronic] United States
• PMID: 22379066 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Outer Membrane Proteins
• Bacterial Toxins
• Bacterial Vaccines
• Enterotoxins
• Escherichia coli Proteins
• LipL32 protein, Leptospira
• Lipoproteins
• Vaccines, Subunit
• Vaccines, Synthetic
• heat-labile enterotoxin, E coli

Topics

• Animals
• Bacterial Outer Membrane Proteins (administration & dosage, immunology)
• Bacterial Toxins (administration & dosage, immunology)
• Bacterial Vaccines (administration & dosage, immunology)
• Cricetinae
• Disease Models, Animal
• Enterotoxins (administration & dosage, immunology)
• Escherichia coli Proteins (administration & dosage, immunology)
• Female
• Leptospira interrogans (immunology)
• Leptospirosis (immunology, mortality, prevention & control)
• Lipoproteins (administration & dosage, immunology)
• Survival Analysis
• Vaccination (methods)
• Vaccines, Subunit (administration & dosage, immunology)
• Vaccines, Synthetic (administration & dosage, immunology)