B2A peptide induces chondrogenic differentiation in vitro and enhances cartilage repair in rats.

Abstract

This study investigated whether the synthetic peptide B2A (B2A2-K-NS) could induce in vitro chondrogenic differentiation and enhance the in vivo repair of damaged cartilage in an osteoarthritis model. In vitro, micromass cultures of murine and human stem cells with and without B2A were used as models of chondrogenic differentiation. Micromasses were evaluated for gene expression using microarray analysis and quantitative PCR; and for extracellular matrix production by Alcian blue staining for sulfated glycosaminoglycan and immunochemical detection of collagen type II. In vivo, osteoarthritis was chemically induced in knees of adult rats by an injection of mono-iodoacetate (MIA) into the synovial space. Treatment was administered at 7- and 14 days after the MIA by injection into the synovial space of B2A or saline and terminated at 21 days, after which knee cartilage damage was determined and scored by histological analysis. In murine C3H10T1/2 micromass culture, B2A induced the expression of more than 11 genes associated with growth factors/receptors, transcription, and the extracellular matrix, including PDGF-AA. B2A also significantly increased the sulfated glycosaminoglycan and collagen of murine- and human micromass cultures. In the knee osteoarthritis model, B2A treatment enhanced cartilage repair compared to untreated knees as determined histologically by a decrease in damage indicators. These findings suggest that B2A induces stem cells chondrogenic differentiation in vitro and enhances cartilage repair in vivo. The results suggest that B2A might be useful to promote cartilage repair.

• Authors: Xinhua Lin, Shobana Shanmugasundaram, Yi Liu, Alexandrine Derrien, Maria Nurminskaya, Paul O Zamora
• Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res) Vol. 30 Issue 8 Pg. 1221-8 (Aug 2012) ISSN: 1554-527X [Electronic] United States
• PMID: 22271086 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Orthopaedic Research Society.

Chemical References

• B2A2-K-NS
• Collagen Type II
• Glycosaminoglycans
• Intercellular Signaling Peptides and Proteins
• Peptides
• A73025
• Iodoacetic Acid

Topics

• Animals
• Cartilage (drug effects, physiology)
• Cell Differentiation (drug effects)
• Cell Line
• Chondrocytes (drug effects)
• Chondrogenesis (drug effects, genetics)
• Collagen Type II (biosynthesis)
• Extracellular Matrix (metabolism)
• Glycosaminoglycans (biosynthesis)
• Humans
• Intercellular Signaling Peptides and Proteins
• Iodoacetic Acid
• Male
• Mice
• Osteoarthritis, Knee (chemically induced, therapy)
• Peptides (pharmacology, therapeutic use)
• Pilot Projects
• Rats