Resveratrol reduces TNF-α-induced U373MG human glioma cell invasion through regulating NF-κB activation and uPA/uPAR expression.

Abstract

BACKGROUND:

High invasiveness of glioma cells is one of the reasons that patients with malignant glioma have a poor prognosis. Resveratrol, a plant compound abundant in the peel of grapes, has been suggested as a potential cancer chemopreventive agent. Therefore, we investigated the effect of resveratrol on glioma cell invasion.

MATERIALS AND METHODS:

The effect of resveratrol on U373MG human glioma cell invasion was assessed by Matrigel assay and methylthiazoltetrazolium assay. Western blotting and reverse transcription-polymerase chain reaction were performed to elucidate the action mechanism of resveratrol.

RESULTS:

Resveratrol reduced tumor necrosis factor (TNF)-α-induced U373MG human glioma cell invasion. In addition, resveratrol repressed nuclear factor kappa B (NF-κB) activation and down-regulated mRNA expression of urokinase plasminogen activator (uPA) and its receptor in TNF-α-treated glioma cells.

CONCLUSION:

These findings suggest that resveratrol could prevent glioma cell invasion via inhibiting proteolysis of extracellular matrix.

• Authors: Jinhyun Ryu, Bo Mi Ku, Yeon Kyung Lee, Joo Yeon Jeong, Seokmin Kang, Jungil Choi, Yeongae Yang, Dong Hoon Lee, Gu Seob Roh, Hyun Joon Kim, Gyeong Jae Cho, Wan Sung Choi, Nayoung Kim, Sang Soo Kang
• Journal: Anticancer research (Anticancer Res) Vol. 31 Issue 12 Pg. 4223-30 (Dec 2011) ISSN: 1791-7530 [Electronic] Greece
• PMID: 22199285 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Drug Combinations
• Laminin
• NF-kappa B
• PLAUR protein, human
• Proteoglycans
• RNA, Messenger
• Receptors, Urokinase Plasminogen Activator
• Stilbenes
• Tumor Necrosis Factor-alpha
• matrigel
• Collagen
• Urokinase-Type Plasminogen Activator
• Resveratrol

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Cell Line, Tumor
• Cell Survival
• Collagen (chemistry)
• Drug Combinations
• Gene Expression Regulation, Neoplastic
• Glioma (pathology)
• Humans
• Laminin (chemistry)
• NF-kappa B (metabolism)
• Neoplasm Invasiveness
• Proteoglycans (chemistry)
• RNA, Messenger (metabolism)
• Receptors, Urokinase Plasminogen Activator (metabolism)
• Resveratrol
• Stilbenes (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)
• Urokinase-Type Plasminogen Activator (metabolism)