Analysis for the combination expression of CK20, FABP1 and MUC2 is sensitive for the prediction of peritoneal recurrence in gastric cancer.

Abstract

Prediction of peritoneal recurrence in gastric cancer patients is important for application of adjuvant chemotherapy. After surgery, occasional patients have peritoneal recurrence despite negative cytology of the peritoneal washings. Thus, molecular detection of a subliminal number of cancer cells in peritoneal washings may overcome the sensitivity limitation of conventional cytology. In this study, expressions of five specific marker genes, namely, TFF1, TFF2, CK20, FABP1 and MUC2, were evaluated for their usefulness as markers of micro-dissemination. It was found that reverse transcriptase-polymerase chain reaction for these five genes yielded results highly specific for the depth of invasion and disease stage. Furthermore, the expression of CK20, FABP1 and MUC2 was a reliable prognostic indicator of peritoneal metastasis. Our results suggest that evaluation of the expression of CK20, FABP1 and MUC2 in peritoneal washings is a useful tool for identifying patients at high risk of peritoneal recurrence who may need adjuvant chemotherapy.

• Authors: Yumiko Satoh, Kazuhiko Mori, Kentaro Kitano, Joji Kitayama, Hiromitsu Yokota, Hiroki Sasaki, Hiroshi Uozaki, Masashi Fukayama, Yasuhiro Seto, Hirokazu Nagawa, Yutaka Yatomi, Daiya Takai
• Journal: Japanese journal of clinical oncology (Jpn J Clin Oncol) Vol. 42 Issue 2 Pg. 148-52 (Feb 2012) ISSN: 1465-3621 [Electronic] England
• PMID: 22172348 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinoembryonic Antigen
• FABP1 protein, human
• Fatty Acid-Binding Proteins
• Keratin-20
• MUC2 protein, human
• Mucin-2
• Peptides
• RNA, Messenger
• TFF1 protein, human
• TFF2 protein, human
• Trefoil Factor-1
• Trefoil Factor-2
• Tumor Suppressor Proteins

Topics

• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Carcinoembryonic Antigen (metabolism)
• Disease-Free Survival
• Fatty Acid-Binding Proteins (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• Kaplan-Meier Estimate
• Keratin-20 (genetics, metabolism)
• Mucin-2 (genetics, metabolism)
• Neoplasm Invasiveness
• Peptides (metabolism)
• Peritoneal Neoplasms (metabolism, mortality, prevention & control, secondary)
• Predictive Value of Tests
• RNA, Messenger (metabolism)
• Recurrence
• Reverse Transcriptase Polymerase Chain Reaction
• Risk Assessment
• Risk Factors
• Sensitivity and Specificity
• Stomach Neoplasms (drug therapy, metabolism, mortality, pathology)
• Trefoil Factor-1
• Trefoil Factor-2
• Tumor Suppressor Proteins (metabolism)