Abstract | INTRODUCTION: METHODS: Radiosynthesis of [(18)F]- scyllo-inositol was automated using a commercial synthesis module. Tumour, inflammation and normal tissue uptakes were evaluated by biodistribution studies and positron emission tomography (PET) imaging using [(18)F]- scyllo-inositol and [(18)F]-FDG in mice bearing subcutaneous MDA-MB-231, MCF-7 and MDA-MB-361 human BC xenografts, intracranial U-87 MG glioma xenografts and turpentine-induced inflammation. RESULTS: The radiosynthesis of [(18)F]- scyllo-inositol was automated with good radiochemical yields (24.6%±3.3%, uncorrected for decay, 65±2 min, n=5) and high specific activities (≥195 GBq/μmol at end of synthesis). Uptake of [(18)F]- scyllo-inositol was greatest in MDA-MB-231 BC tumours and was comparable to that of [(18)F]-FDG (4.6±0.5 vs. 5.5±2.1 %ID/g, respectively; P=.40), but was marginally lower in MDA-MB-361 and MCF-7 xenografts. Uptake of [(18)F]- scyllo-inositol in inflammation was lower than [(18)F]-FDG. While uptake of [(18)F]- scyllo-inositol in intracranial U-87 MG xenografts was significantly lower than [(18)F]-FDG, the tumour-to-brain ratio was significantly higher (10.6±2.5 vs. 2.1±0.6; P=.001). CONCLUSIONS: Consistent with biodistribution studies, uptake of [(18)F]- scyllo-inositol was successfully visualized by PET imaging in human BC and glioma xenografts, with lower accumulation in inflammatory tissue than [(18)F]-FDG. The tumour-to-brain ratio of [(18)F]- scyllo-inositol was also significantly higher than that of [(18)F]-FDG for visualizing intracranial glioma xenografts in NOD SCID mice, giving a better contrast.
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Authors | Kristin McLarty, Matthew D Moran, Deborah A Scollard, Conrad Chan, Nesrin Sabha, Joydeep Mukherjee, Abhijit Guha, JoAnne McLaurin, Mark Nitz, Sylvain Houle, Alan A Wilson, Raymond M Reilly, Neil Vasdev |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 38
Issue 7
Pg. 953-9
(Oct 2011)
ISSN: 1872-9614 [Electronic] United States |
PMID | 21982567
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- (18F)-1-deoxy-1-fluoro-scyllo-inositol
- Fluorodeoxyglucose F18
- Inositol
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Topics |
- Animals
- Biological Transport
- Brain Neoplasms
(diagnostic imaging, metabolism, pathology)
- Breast Neoplasms
(diagnostic imaging, metabolism, pathology)
- Cell Line, Tumor
- Female
- Fluorodeoxyglucose F18
(chemistry, metabolism)
- Humans
- Inflammation
(diagnostic imaging, metabolism, pathology)
- Inositol
(analogs & derivatives, chemistry, metabolism)
- Male
- Mice
- Mice, Inbred NOD
- Mice, Nude
- Mice, SCID
- Neoplasm Transplantation
- Positron-Emission Tomography
(methods)
- Radiochemistry
- Transplantation, Heterologous
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