Abstract | AIM: METHODS: Six-week-old LDLR-deficient mice were fed an atherogenic high-fat diet for 17 weeks and then randomly divided into two groups. One group was administered darapladib (50 mg·kg(-1)·d(-1); po) for 6 weeks. The other group was administered saline as a control. Serum lipid levels were measured using the corresponding kits, and three inflammatory markers--interleukin-6 (IL-6), C reactive protein ( hs-CRP), and platelet activating factor (PAF)--were determined using ELISA. Atherosclerotic plaque areas were stained with Sudan IV, and inflammatory gene expression at the lesions was evaluated using quantitative real-time PCR. RESULTS: The body weight and serum lipid level between the two groups were similar at the end of the dietary period. The serum lp-PLA2 activity, hs-CRP and IL-6 levels, however, were significantly reduced in the darpladib group. The inhibition of lp-PLA2 did not alter the serum PAF level. Furthermore, the plaque area, from the aortic arch to the abdominal aorta, was significantly reduced in the darpladib group. Additionally, the expression of inflammatory genes monocyte chemotactic protein-1 (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1) was significantly reduced at the lesions in the darapladib group. CONCLUSION:
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Authors | Miao-miao Hu, Jie Zhang, Wen-yi Wang, Wen-yu Wu, Yan-ling Ma, Wei-hai Chen, Yi-ping Wang |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 32
Issue 10
Pg. 1253-8
(Oct 2011)
ISSN: 1745-7254 [Electronic] United States |
PMID | 21970837
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzaldehydes
- Chemokine CCL2
- Enzyme Inhibitors
- Interleukin-6
- Oximes
- Receptors, LDL
- Vascular Cell Adhesion Molecule-1
- C-Reactive Protein
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
- darapladib
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Topics |
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
(antagonists & inhibitors, metabolism)
- Animals
- Atherosclerosis
(drug therapy, genetics, immunology, metabolism)
- Benzaldehydes
(pharmacology)
- C-Reactive Protein
(immunology)
- Chemokine CCL2
(immunology)
- Enzyme Inhibitors
(pharmacology)
- Gene Deletion
- Interleukin-6
(immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Oximes
(pharmacology)
- Plaque, Atherosclerotic
(drug therapy)
- Receptors, LDL
(genetics)
- Vascular Cell Adhesion Molecule-1
(immunology)
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