Defective dendritic cell generation from monocytes is a potential reason for poor therapeutic efficacy of interferon α2b (IFNα2b) in cervical cancer.

Abstract

Despite being a pleiotropic cytokine, the therapeutic potential of interferon α2b (IFNα2b) is debatable. Thus, the need for identifying predictive marker(s) for patients who are most likely to benefit from the treatment is pivotal for avoiding the exposure of nonresponsive patients to the toxicity of the treatment. To account for the attenuated efficacy of the drug, we have verified its dendritic cell (DC) maturating ability from monocytes of cervical cancer stage IIIB (CaCx-IIIB) patients. First, we evaluated the status of monocytes from CaCx-IIIB and healthy women by conducting flow cytometric studies of various activation markers and a cytokine analysis by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. Immature DCs were then generated from these monocytes and matured with low-dose IFNα2b (1500 units/mL). A functional and phenotypic comparative analysis of these matured DCs was performed by flow cytometric, proliferative, cytotoxic, and enzyme-linked immunosorbent assays. Our study shows that monocytes isolated from CaCx-IIIB are impaired, and in vitro maturation with IFNα2b did not significantly improve the functional repertoire of DCs generated from these monocytes in comparison with healthy controls. This impairment of monocytes might be a plausible reason for the attenuated efficacy of this drug alone in treating CaCx-IIIB patients, and this imbalance of immune parameters associated with the stage of malignancy might be considered an effective marker to design a proper therapeutic regimen.

• Authors: Soumyabrata Roy, Shyamal Goswami, Anamika Bose, Kuntal Kanti Goswami, Koustav Sarkar, Krishnendu Chakraborty, Tathagata Chakraborty, Smarajit Pal, Atanu Haldar, Parthasarathi Basu, Jaydip Biswas, Rathindranath Baral
• Journal: Translational research : the journal of laboratory and clinical medicine (Transl Res) Vol. 158 Issue 4 Pg. 200-13 (Oct 2011) ISSN: 1878-1810 [Electronic] United States
• PMID: 21925117 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Mosby, Inc. All rights reserved.

Chemical References

• Cytokines
• Interferon alpha-2
• Interferon-alpha
• Recombinant Proteins
• Receptor, Interferon alpha-beta

Topics

• Adult
• Aged
• Antigen Presentation (drug effects)
• CD8-Positive T-Lymphocytes (immunology)
• Case-Control Studies
• Cell Differentiation (drug effects, immunology)
• Cytokines (biosynthesis)
• Dendritic Cells (drug effects, immunology, pathology)
• Female
• Humans
• In Vitro Techniques
• Interferon alpha-2
• Interferon-alpha (adverse effects, therapeutic use)
• Lymphocyte Activation
• Middle Aged
• Monocytes (drug effects, immunology, pathology)
• Receptor, Interferon alpha-beta (metabolism)
• Recombinant Proteins
• Translational Research, Biomedical
• Treatment Failure
• Uterine Cervical Neoplasms (drug therapy, immunology, pathology)