Protective effects of ω-conotoxin on amyloid-β-induced damage in PC12 cells.

Abstract

The present study was designed to investigate the potential neuroprotective effect of ω-conotoxin (ω-CTX) in a cell-based model of Alzheimer's disease (AD) using cultured PC12 cells incubated with β-amyloid (Aβ). Immunohistochemical staining, Western blot, MTT and TdT-mediated dUTP-biotin nick end labeling (TUNEL) analysis were employed to assess the cell viability and cell death. Aβ in this model was clearly neurotoxic, inducing necrosis and apoptosis. ω-CTX antagonized the effects of Aβ: there was an increase in cell viability and a suppression of inflammatory- and oxidative stress-related factors. These data suggest that ω-CTX may have neuroprotective actions against Aβ-induced neurotoxicity. The significance of these new findings relative to the etiology and treatment of AD is discussed.

• Authors: Marong Fang, Jing Wang, Shu Han, Zhiying Hu, Jin-biao Zhan, Shucai Ling, John A Rudd, Yu Geng
• Journal: Toxicology letters (Toxicol Lett) Vol. 206 Issue 3 Pg. 325-38 (Oct 30 2011) ISSN: 1879-3169 [Electronic] Netherlands
• PMID: 21839817 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Bax protein, rat
• Neuroprotective Agents
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• omega-Conotoxins
• Caspase 3
• Trypan Blue

Topics

• Amyloid beta-Peptides (toxicity)
• Animals
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Immunohistochemistry
• Microscopy, Phase-Contrast
• Neuroprotective Agents (pharmacology)
• Oxidative Stress (drug effects)
• PC12 Cells
• Proto-Oncogene Proteins c-bcl-2 (analysis)
• Rats
• Trypan Blue (metabolism)
• bcl-2-Associated X Protein (analysis)
• omega-Conotoxins (pharmacology)